Format

Send to

Choose Destination
  • Filters activated: Field: Title Word. Clear all
Anticancer Res. 2017 Dec;37(12):6619-6628.

Antiproliferative Activity and Induction of Apoptosis in Human Melanoma Cells by Houttuynia cordata Thunb Extract.

Author information

1
School of Anti-Aging and Regenerative Medicine, Mae Fah Luang University, Bangkok, Thailand.
2
Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
3
Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand salunya.tan@mahidol.edu.

Abstract

BACKGROUND/AIM:

To analyze the apoptotic effect of Houttuynia cordata Thunb (HCT) extract on human melanoma A375 cells and its underlying mechanisms.

MATERIALS AND METHODS:

The effects of HCT on cell death were determined using the MTT assay. Hoechst 33342 staining was conducted to confirm the detection of cell apoptosis. Caspase-3 and caspase-8 mRNA and cleaved protein levels were investigated by RT-PCR and western blotting, respectively. The release of high mobility group box 1 (HMGB1) and phosphorylation of mitogen-activated protein kinase (MAPK) were determined by ELISA.

RESULTS:

Caspase-3 and caspase-8 specific inhibitors suppressed HCT-induced cell death. HCT increased caspase-3 and caspase-8 mRNA, protein levels, and caspase activities in a concentration- and time-dependent manner. HCT induced MAPK phosphorylation in a time-dependent fashion. Pretreatment of cells with a selective inhibitor of p38 MAPK reduced apoptosis and reversed the levels of HMGB1 release in response to HCT treatment.

CONCLUSION:

HCT induces A375 programmed cell death by activating the caspase-dependent pathway and by p38 phosphorylation associated with HMGB1 reduction.

KEYWORDS:

Houttuynia cordata Thunb; apoptosis; caspase; high mobility group box 1; melanoma; mitogen-activated protein kinase

PMID:
29187437
DOI:
10.21873/anticanres.12119
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center