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J Biomed Mater Res A. 2017 Oct;105(10):2830-2842. doi: 10.1002/jbm.a.36135. Epub 2017 Jul 14.

G-CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo.

Author information

1
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
2
Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3
Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran.
4
Departments of Dermatology and Pathology, Tehran University of Medical Sciences, Razi Hospital, Tehran, Iran.
5
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.

Abstract

Sustained release of functional growth factors can be considered as a beneficial methodology for wound healing. In this study, recombinant human granulocyte colony-stimulating factor (G-CSF)-loaded chitosan nanoparticles were incorporated in Poly(ε-caprolactone) (PCL) nanofibers, followed by surface coating with collagen type I. Physical and mechanical properties of the PCL nanofibers containing G-CSF loaded chitosan nanoparticles PCL/NP(G-CSF) and in vivo performance for wound healing were investigated. G-CSF structural stability was evaluated through SDS_PAGE, reversed phase (RP) HPLC and size-exclusion chromatography, as well as circular dichroism. Nanofiber/nanoparticle composite scaffold was demonstrated to have appropriate mechanical properties as a wound dresser and a sustained release of functional G-CSF. The PCL/NP(G-CSF) scaffold showed a suitable proliferation and well-adherent morphology of stem cells. In vivo study and histopathological evaluation outcome revealed that skin regeneration was dramatically accelerated under PCL/NP(G-CSF) as compared with control groups. Superior fibroblast maturation, enhanced collagen deposition and minimum inflammatory cells were also the beneficial properties of PCL/NP(G-CSF) over the commercial dressing. The synergistic effect of extracellular matrix-mimicking nanofibrous membrane and G-CSF could develop a suitable supportive substrate in order to extensive utilization for the healing of skin wounds.

KEYWORDS:

G-CSF; nanofiber; nanoparticles; skin; wound dressing

PMID:
28589686
DOI:
10.1002/jbm.a.36135
[Indexed for MEDLINE]

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