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Mater Sci Eng C Mater Biol Appl. 2017 Jan 1;70(Pt 1):317-326. doi: 10.1016/j.msec.2016.09.025. Epub 2016 Sep 13.

P(TA) macro-, micro-, nanoparticle-embedded super porous p(HEMA) cryogels as wound dressing material.

Author information

1
Faculty of Science & Arts, Chemistry Department, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey; Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey. Electronic address: sahiner71@gmail.com.
2
Faculty of Science & Arts, Chemistry Department, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey.
3
Department of Leather Engineering, Ege University, Bornova, 35100 Izmir, Turkey.
4
School of Medicine, Department of Pharmacology, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100, Turkey.

Abstract

Super porous poly(2-hydroxy ethyl methacrylate) (p(HEMA)) cryogel was successfully synthesized by using polyethylene glycol diacrylate (p(EGDA)) crosslinker under cryogenic conditions. Poly(Tannic acid) (p(TA)) macro-, micro-, and nanoparticles prepared from a natural polyphenol, tannic acid (TA), were embedded into p(HEMA) cryogel networks to obtain composite p(TA) particle-embedded p(HEMA) cryogel. Different size ranges of spherical p(TA) particles, 2000-500μm, 500-200μm, 200-20μm, and 20-0.5μm size, were included in the cryogel network and illustrated by digital camera, optic microscope, and SEM images of the microgel-cryogel network. The swelling properties and moisture content of p(TA) microgel-embedded p(HEMA) cryogel were investigated at wound healing pH conditions such as pH5.4, 7.4, and 9 at 37.5°C, and the highest swelling capacity was found at pH9 with 972±2% swelling in 30s. Higher amounts of DI water were quickly absorbed by p(HEMA)-based cryogel, and moisture retention within the cryogel structure for a longer time period at room temperature is due to existence of p(TA) particles. Degradation profiles of p(TA) particle-embedded p(HEMA) cryogel were shown to be controlled by different pH conditions, and a linear release profile was found with total cumulative release of 5.8±0.8mg/g TA up to 12days at pH7.4 and 37.5°C. The antioxidant behavior of degraded p(TA) particles from p(HEMA) cryogel were found as 46±1μgmL-1 gallic acid equivalent and 165±18mMtroloxequivalentg-1. The p(TA) particle-embedded p(HEMA) cryogel has high hemocompatibility with 0.0158±0.0126% hemolysis ratio, and effective hemostatic properties with 8.1±0.9 blood clotting index.

KEYWORDS:

Antioxidant; Hemocompatible wound dressing/covering network; Macroporous cryogel/hydrogel; Microgel embedded cryogel composites; Tannic acid microgel

PMID:
27770897
DOI:
10.1016/j.msec.2016.09.025
[Indexed for MEDLINE]

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