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J Vis Exp. 2008 Dec 11;(22). pii: 1029. doi: 10.3791/1029.

Preparation of single-cell suspensions from mouse spleen with the gentleMACS Dissociator.

Author information

1
Miltenyi Biotec, GmbH.

Abstract

Single-cell suspensions are a prerequisite for experiments in cell separation, cell analysis and cell culture. To avoid tedious and often painful manual dissociations the gentleMACS Dissociator allows one to dissociate tissue very efficiently under controlled and reproducible conditions. The gentleMACS Dissociator can optimally dissociate mouse spleen, combining timesaving and standardization with user-safety. This video describes how to dissociate mouse spleens using the gentleMACS Dissociator, an automated bench-top device that can mechanically disrupt tissues using special tubes to produce viable cell suspensions. Following dissociation, spleens are filtered, centrifuged, and resuspended for further applications.

PMID:
19229170
PMCID:
PMC2762924
DOI:
10.3791/1029
[Indexed for MEDLINE]
Free PMC Article

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