Format

Send to

Choose Destination
  • Filters activated: Field: Title Word. Clear all
Carbohydr Polym. 2018 Nov 1;199:593-602. doi: 10.1016/j.carbpol.2018.07.057. Epub 2018 Jul 18.

3D-printed chitosan-based scaffolds: An in vitro study of human skin cell growth and an in-vivo wound healing evaluation in experimental diabetes in rats.

Author information

1
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124, Parma, Italy; Department of Microbiology & Immunology, University of Otago, Dunedin 9054, New Zealand.
2
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124, Parma, Italy. Electronic address: lisa.elviri@unipr.it.
3
Department of Chemistry, University of Otago, Dunedin 9054, New Zealand.
4
Department of Microbiology & Immunology, University of Otago, Dunedin 9054, New Zealand.
5
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124, Parma, Italy.
6
Biopharmanet TEC, University of Parma, Parco Area delle Scienze 27/A, 43124, Parma, Italy.
7
Department of Medicine and Surgery, University of Parma, 43124, Parma, Italy.

Abstract

The fabrication of porous 3D printed chitosan (CH) scaffolds for skin tissue regeneration and their behavior in terms of biocompatibility, cytocompatibility and toxicity toward human fibroblasts (Nhdf) and keratinocytes (HaCaT), are presented and discussed. 3D cell cultures achieved after 20 and 35 days of incubation showed significant in vitro qualitative and quantitative cell growth as measured by neutral red staining and MTT assays and confirmed by scanning electron microphotographs. The best cell growth was obtained after 35 days on 3D scaffolds when the Nhdf and HaCaT cells, seeded together, filled the pores in the scaffolds. An early skin-like layer consisting of a mass of fibroblast and keratinocyte cells growing together was observed. The tests of 3D printed scaffolds in wound healing carried out on streptozotocin-induced diabetic rats demonstrate that 3D printed scaffolds improve the quality of the restored tissue with respect to both commercial patch and spontaneous healing.

KEYWORDS:

3D printing; Chitosan biocompatibility; Chitosan scaffold; Diabetic induced rats; Fibroblast and keratinocyte cells; Skin tissue engineering

PMID:
30143167
DOI:
10.1016/j.carbpol.2018.07.057
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center