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Int J Biol Macromol. 2018 Dec;120(Pt A):288-296. doi: 10.1016/j.ijbiomac.2018.08.061. Epub 2018 Aug 14.

Structural characterization and anti-A549 lung cancer cells bioactivity of a polysaccharide from Houttuynia cordata.

Author information

1
Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Pudong, Shanghai 201203, University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
2
Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Pudong, Shanghai 201203, University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China.
3
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: yumei@mail.hzau.edu.cn.
4
Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Pudong, Shanghai 201203, University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China. Electronic address: dingkan@simm.ac.cn.

Abstract

A water-soluble pectic polysaccharide HCA4S1 was isolated from Houttuynia cordata and purified by DEAE Cellulose and Sephacryl S-300 column. HCA4S1 with an average molecular weight of 21.7 kDa mainly consisted of rhamnose, galacturonic acid, galactose, and arabinose. By using partial acid hydrolysis, methylation analysis, and NMR spectra, the structure of this polysaccharide is found to have a backbone consisting of 1,4-linked α‑d‑GalA and 1,2,4-linked α‑l‑Rha. The latter was substituted at C-4 position by 1,4 linked, 1,6-linked β‑Galp, or Teminal linked β‑Gal. Bioactivity test showed that this polysaccharide might inhibit the proliferation of A549 lung cancer cell by inducing cell cycle arrest and apoptosis. The expression of cleaved caspase 3 and cyclinB1 was observed to be upregulated after the treatment with this polysaccharide. Collectively, these results suggest that the pectin HCA4S1 from Houttuynia cordata is of potential value in the treatment of lung cancer, though the underlying mechanisms remain to be further confirmed.

KEYWORDS:

Anti-cancer; Houttuynia cordata; Pectin

PMID:
30114425
DOI:
10.1016/j.ijbiomac.2018.08.061
[Indexed for MEDLINE]

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