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Acta Biomater. 2015 Jul;20:82-93. doi: 10.1016/j.actbio.2015.03.029. Epub 2015 Apr 1.

Surface charge tunability as a powerful strategy to control electrostatic interaction for high efficiency silencing, using tailored oligopeptide-modified poly(beta-amino ester)s (PBAEs).

Author information

1
Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain.
2
Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain. Electronic address: victor.ramos@iqs.url.edu.
3
Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain. Electronic address: salvador.borros@iqs.url.edu.

Abstract

Here we present an extended family of pBAEs that incorporate terminal oligopeptide moieties synthesized from both positive and negative amino acids. Polymer formulations of mixtures of negative and positive oligopeptide-modified pBAEs are capable of condensing siRNA into discrete nanoparticles. We have demonstrated that efficient delivery of nucleic acids in a cell-type dependent manner can be achieved by careful control of the pBAE formulation. In addition, our approach of adding differently charged oligopeptides to the termini of poly(β-amino ester)s is of great interest for the design of tailored complexes having specific features, such as tuneable zeta potential. We anticipate that this surface charge tunability may be a powerful strategy to control unwanted electrostatic interactions, while preserving high silencing efficiency and reduced toxicity.

KEYWORDS:

Nanoparticles; Non viral systems; Poly(beta-amino ester)s; Polyplexes; SiRNA

PMID:
25839122
DOI:
10.1016/j.actbio.2015.03.029
[Indexed for MEDLINE]

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