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Mol Biochem Parasitol. 1989 Jul;35(3):277-83.

Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos.

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Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH.


High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.

[Indexed for MEDLINE]

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