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Curr Opin Mol Ther. 2003 Jun;5(3):217-24.

siRNAs and shRNAs: skeleton keys to the human genome.

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Watson School of Biological Sciences, Cold Spring Harbor Laboratory, McClintock Building, 1 Bungtown Road, Cold Spring Harbor, NY 1172, USA.


The use of RNA interference (RNAi) to evoke gene silencing in mammalian cell systems is rapidly becoming a standard laboratory technique. Recent experiments have also proven the utility of RNAi in animals. The next few years will bear witness to the first RNAi-based genome-wide screens in mammals, breaking the barrier to the use of mammalian cells as a genetic system. In this review, some of the key features of RNAi in mammalian systems are discussed.

[Indexed for MEDLINE]

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