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1.
Medicine (Baltimore). 2019 Sep;98(39):e17386. doi: 10.1097/MD.0000000000017386.

The efficacy and safety of polydeoxyribonucleotide for the treatment of knee osteoarthritis: Systematic review and meta-analysis of randomized controlled trials.

Author information

1
Department of Orthopaedic Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Abstract

INTRODUCTION:

The purpose of this study was to use meta-analysis techniques to evaluate the efficacy and safety of polydeoxyribonucleotide (PDRN) injections for knee osteoarthritis (OA) treatment.

METHODS:

Multiple comprehensive databases, including MEDLINE, EMBASE, and the Cochrane Library, were searched in November 2018 for studies that compared the effectiveness and safety of intra-articular PDRN injection for the knee joint with hyaluronic acid (HA) injection. Two reviewers independently determined study inclusion and they extracted data using a standardized data extraction form. The predefined primary outcome was Visual Analogue Scale. Secondary outcomes included Knee Injury and Osteoarthritis Outcome Score (KOOS), Knee Society Score (KSS), and adverse events.

RESULTS:

Five randomized controlled trials were included in the meta-analysis. After 1 and 2 months, patients in the PDRN group showed significantly better improvement in pain than the HA group (P = .04 and P = .02, respectively). There was no significant difference in pain after 4 months. The pooled analysis showed that no significant differences were seen in function (KOOS and KSS) scores between the PDRN and HA groups (all P > .05) at all time points. There was no significant difference in adverse events between 2 groups (relative risks = 2.15, 95% confidential interval: 0.17-26.67, P = .55).

CONCLUSION:

The intra-articular use of PDRN was similar in function to HA, and the pain-relief effect was superior to HA for 2 months post-injection. Therefore, it could be a favorable alternative to HA to treat persistent pain associated with knee OA while avoiding side effects.Level of evidence I.

PMID:
31574892
DOI:
10.1097/MD.0000000000017386
[Indexed for MEDLINE]
Free PMC Article
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2.
Anticancer Res. 2019 Oct;39(10):5541-5549. doi: 10.21873/anticanres.13747.

Linking Infection and Prostate Cancer Progression: Toll-like Receptor3 Stimulation Rewires Glucose Metabolism in Prostate Cells.

Author information

1
Department of Biochemical Sciences A. Rossi Fanelli, Sapienza University of Rome, Rome, Italy.
2
Department of Biochemical Sciences A. Rossi Fanelli, Sapienza University of Rome, Rome, Italy francesca.cutruzzola@uniroma1.it.

Abstract

BACKGROUND/AIM:

The connection between prostate cancer and inflammation has been proposed many years ago, but very little is known about the metabolic adaptations of prostate cells in case of infection or inflammation. The aim of this study was to examine the effect of the stimulation of Toll-like receptor 3 (TLR3) on the metabolism of prostate cancer (PCa) cell lines and benign prostate cells.

MATERIALS AND METHODS:

Cytofluorimetry, qRT-PCR, western blot and Gas-chromatography/Mass-spectrometry were used.

RESULTS:

Reprogramming of glucose utilization involving hypoxia-inducible factor 1-alpha (HIF-1α) and the extracellular adenosine axis was observed. TLR3 stimulation synergized with adenosine receptor A2b on PCa cells, and induced a strong production of lactate, exacerbating the Warburg effect. Moreover, stimulation of benign prostate cells with poly I:C reduced lactate secretion, a characteristic typical of the neoplastic transformation.

CONCLUSION:

TLR3 stimulation promotes metabolic adaptations likely involved in the mechanisms of disease onset and progression.

KEYWORDS:

A2b; CD73; Inflammation; Warburg effect; adenosine; metabolism

PMID:
31570448
DOI:
10.21873/anticanres.13747
[Indexed for MEDLINE]
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3.
Fa Yi Xue Za Zhi. 2019 Aug;35(4):387-392. doi: 10.12116/j.issn.1004-5619.2019.04.001. Epub 2019 Aug 25.

Quantitative Expression of RNA from Frozen Organs and Formaldehyde-fixed and Paraffin-embedded Tissues.

[Article in Chinese, English; Abstract available in Chinese from the publisher]
Lü YH1,2,3, Li SY2, Li ZH1, Tao RY2,3, Shao Y2, Hu Q1, Yang ZF1, Chen YJ2,3.

Author information

1
School of Basic Medical Sciences, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.
2
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
3
West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610065, China.

Abstract

Objective Quantitative analysis and comparison of the expression of ribonucleic acid (RNA) from frozen organs and formaldehyde-fixed and paraffin-embedded (FFPE) tissues. Methods Frozen specimens of human brain, myocardium and liver tissues as well as FFPE samples at different postmortem intervals were collected and mass concentration of RNA was extracted and detected. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technology was used to analyze the amplification efficiency and relative expression of each RNA marker. Results The mass concentration and integrity of RNA extracted from FFPE samples were relatively low compared with frozen specimens. The amplification efficiency of RNA markers was related with RNA species and the length of amplification products. Among them, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) with relatively long amplification products failed to achieve optimal amplification efficiency, whereas 5S ribosomal RNA (5S rRNA) achieved ideal amplification efficiency and showed quite stable expression across various tissues, therefore it was chosen as internal reference marker. The expression quantity of GAPDH and ACTB in frozen specimens with longer postmortem intervals and in FFPE samples with relatively long amplification products was decreased. The expressions of tissue-specific microRNAs (miRNAs), GAPDH and ACTB with relatively short amplification products had consistency in the same tissues and FFPE samples. Conclusion Through standardizing the RT-qPCR experiment, selecting the appropriate RNA marker and designing primers of appropriate product length, RNA expression levels of FFPE samples can be accurately quantified.

KEYWORDS:

forensic pathology; ribonucleic acid; paraffin embedding; freeze preservation; reverse transcription-polymerase chain reaction; ribonucleic acid integrity number

[Indexed for MEDLINE]
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Conflict of interest statement

The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

4.
Arch Virol. 2019 Nov;164(11):2761-2768. doi: 10.1007/s00705-019-04394-8. Epub 2019 Sep 10.

Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus.

Author information

1
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
2
College of Global Change and Earth System Science, Beijing Normal University, Beijing, 100875, China.
3
Jilin Institute of Animal Husbandry and Veterinary Science, Changchun, 130062, Jilin, China.
4
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China. huguixue901103@126.com.

Abstract

A multiplex polymerase chain reaction (mPCR) assay was developed to detect and distinguish feline panleukopenia virus (FPV), feline bocavirus (FBoV) and feline astrovirus (FeAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of the three viruses and were used to specifically amplify targeted fragments of 237 bp from the VP2 gene of FPV, 465 bp from the NP1 gene of FBoV and 645 bp from the RdRp gene of FeAstV. The results showed that this mPCR assay was effective, because it could detect at least 2.25-4.04 × 104 copies of genomic DNA of the three viruses per μl, was highly specific, and had a good broad-spectrum ability to detect different genotypes of the targeted viruses. A total of 197 faecal samples that had been screened previously for FeAstV and FBoV were collected from domestic cats in northeast China and were tested for the three viruses using the newly developed mPCR assay. The total positive rate for these three viruses was 59.89% (118/197). From these samples, DNA from FPV, FBoV and FeAstV was detected in 73, 51 and 46 faecal samples, respectively. The mPCR testing results agreed with the routine PCR results with a coincidence rate of 100%. The results of this study show that this mPCR assay can simultaneously detect and differentiate FPV, FBoV and FeAstV and can be used as an easy, specific and efficient detection tool for clinical diagnosis and epidemiological investigation of these three viruses.

PMID:
31506786
DOI:
10.1007/s00705-019-04394-8
[Indexed for MEDLINE]
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5.
Zhonghua Liu Xing Bing Xue Za Zhi. 2019 Aug 10;40(8):1018-1022. doi: 10.3760/cma.j.issn.0254-6450.2019.08.027.

[Application of NASBA and RPA in detection of pathogenic bacteria].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Ningxia Hui Autonomous Region Food Testing and Research Institute, Yinchuan 750001, China.
2
Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Abstract

Nucleic acid sequence-based amplification and recombinase polymerase amplification are the recently developed thermostatic amplification techniques based on PCR. This paper briefly summarizes the principle of reaction, design principle of primer and probe, advantage of these two techniques (simple, accurate, highly sensitive and rapid) and introduces the application of the techniques in the detection of pathogenic bacteria.

KEYWORDS:

Nucleic acid sequence-based amplification; Pathogenic bacteria; Recombinase polymerase amplification

[Indexed for MEDLINE]
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6.
Sheng Wu Gong Cheng Xue Bao. 2019 Aug 25;35(8):1463-1468. doi: 10.13345/j.cjb.190110.

[Prokaryotic expression and transmembrane transfer of fusion protein TAT-RIG-I-GFP].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Shandong Jianmu Biological Pharmaceutical Co., Ltd., Jinan 250100, Shandong, China.
2
Institute of Poultry Science, Shandong Academy of Agricultural Science, Jinan 250100, Shandong, China.

Abstract

We studied the construction of fusion protein TAT-RIG-I-GFP prokaryotic expression vector and verified the function of TAT in transmembrane delivery. First, four pairs of specific primers were designed, and the RIG-I gene of Mallard Duck (Anas platyrhynchos) was cloned. Then, the pET-TAT-RIG-I-GFP and pET-RIG-I-GFP prokaryotic expression vectors were constructed. Meanwhile, they were converted to E. coli BL21 (DE3), which were induced to be expressed after culture. After the purification of His-60 nickel affinity chromatography column and the identification of SDS-PAGE, the purified TAT-RIG-I-GFP and RIG-I-GFP proteins were incubated to DF-1 cells. Finally, fluorescence microscopy was used to observe whether the corresponding fluorescence was produced in DF-1 cells. The results showed that pET-TAT-RIG-I-GFP fusion with TAT showed obvious green fluorescence in DF-1 cells. However, the pET-RIG-I-GFP without TAT cannot display green fluorescence. This shows that TAT-fused protein have successfully delivered DF-1 cells and play a key role in transmembrane delivery. In conclusion, these results provide a solid material basis for further study of antiviral drugs in poultry.

KEYWORDS:

RIG-I; TAT; fusion protein; transmembrane delivery

PMID:
31441617
DOI:
10.13345/j.cjb.190110
[Indexed for MEDLINE]
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7.
Chem Commun (Camb). 2019 Sep 16;55(72):10713-10716. doi: 10.1039/c9cc03587g. Epub 2019 Aug 20.

Red light-triggered nucleic acid-templated reaction based on cyclic oligonucleotide substrates.

Author information

1
Department of Chemistry and Pharmacy, Organic Chemistry II, Friedrich-Alexander-University of Erlangen-Nürnberg (FAU), 91058 Erlangen, Germany. Andriy.Mokhir@fau.de.
2
Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-University, 60438 Frankfurt, Germany.
3
Computer-Chemistry-Center and Interdisciplinary Center for Molecular Materials, Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91052 Erlangen, Germany.

Abstract

A red light-triggered reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by specific nucleic acid templates and generates a bright fluorescent probe was developed. We confirmed that this reaction is compatible with fluorescence correlation spectroscopy (FCS) thereby allowing detection of nucleic acids down to 1 nM.

PMID:
31429427
DOI:
10.1039/c9cc03587g
[Indexed for MEDLINE]
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8.
Bioengineered. 2019 Dec;10(1):345-352. doi: 10.1080/21655979.2019.1652490.

Clinical significance of serum MicroRNA-203 in patients with acute myeloid leukemia.

Author information

1
a Department of Pediatric medicine, Linyi Central Hospital , Yishui , China.

Abstract

This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that serum miR-203 expression was significantly reduced in AML patients compared with healthy controls. Receiver operating characteristic curve (ROC) analysis revealed miR-203 could distinguish AML cases from normal controls. Low serum miR-203 levels were associated with worse clinical features, as well as poorer overall survival and relapse free survival of AML patients. Moreover, multivariate analysis confirmed low serum miR-203 expression to be an independent unfavorable prognostic predictor for AML. The bioinformatics analysis showed that the downstream genes and pathways of miR-203 was closely associated with tumorigenesis. Downregulation of miR-203 in AML cell lines upregulated the expression levels of oncogenic promoters such as CREB1, SRC and HDAC1. Thus, these findings demonstrated that serum miR-203 might be a promising biomarker for the diagnosis and prognosis of AML.

KEYWORDS:

acute myeloid leukemia; diagnosis; prognosis; serum miR-203

PMID:
31411110
PMCID:
PMC6738448
[Available on 2020-08-14]
DOI:
10.1080/21655979.2019.1652490
[Indexed for MEDLINE]
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9.
Klin Lab Diagn. 2019;64(7):413-416. doi: 10.18821/0869-2084-2019-64-7-413-416.

[Study of the quantity of interleukin-6 by SDS-PAAG electrophoresis and immuno-enzyme analysis in mixed saliva after rinsing the oral cavity with oligonucleotide specific.]

[Article in Russian]

Author information

1
Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, 127473, Moscow, Russia.
2
The A.N. Belozersky Institute оf Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia.

Abstract

The selective properties of a solution of oligonucleotide specific to IL-6 on the concentration of IL-6 in mixed saliva of patients with oral inflammatory processes were studied using SDS-PAGE by electrophoresis and enzyme immunoassay. The application of these methods showed that in the mixed saliva of patients after rinsing with a solution of an oligonucleotide specific for IL-6, the amount of IL-6 decreases. The ELISA Kit and 20% SDS-PAGE showed the highest sensitivity to determine the concentration of IL-6 in saliva, which should be considered in clinical laboratory practice.

KEYWORDS:

electrophoresis; enzyme immunoassay analysis; mixed saliva; oligonucleotide solution specific to interleukin-6

[Indexed for MEDLINE]
10.
Chem Commun (Camb). 2019 Aug 22;55(69):10300-10303. doi: 10.1039/c9cc04608a.

Freezing promoted hybridization of very short DNA oligonucleotides.

Author information

1
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China. xuwentao@cau.edu.cn.

Abstract

Shorter DNA probes provide better specificity for hybridization, but they may not form stable duplexes at room temperature. In this study, we used thiazole orange to follow DNA hybridization upon freezing and achieved stable 5-mer duplex DNA. Using multiple short probes in tandem, long DNA could also be studied. This study provides insights into DNA hybridization in the frozen state and expands the application of freezing for nucleic acid chemistry.

PMID:
31397452
DOI:
10.1039/c9cc04608a
[Indexed for MEDLINE]
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11.
Pharm Res. 2019 Aug 8;36(10):145. doi: 10.1007/s11095-019-2669-5.

Anti-GPC3 Antibody Tagged Cationic Switchable Lipid-Based Nanoparticles for the Co-Delivery of Anti-miRNA27a And Sorafenib in Liver Cancers.

Author information

1
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. AlissonAguirretpt@yahoo.com.
2
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Abstract

PURPOSE:

The immediate plasma metabolism and development of chemo-resistance (single agent) severely hampers the clinical effectiveness of Sorafenib (SRF) in liver cancer therapy. MicroRNA27a inhibition is a promising biological strategy for breast cancer therapy.

METHODS:

In this study, we aimed to prepare SRF and anti-miRNA27a-loaded anti-GPC3 antibody targeted lipid nanoparticles to enhance the therapeutic efficacy against liver cancers. In this study, we have employed a unique cationic switchable lipid (CSL) as a mean to encapsulate miRNA as well as to confer pH-responsiveness to the nanocarrier system.

RESULTS:

The G-S27LN was nanosized and offered a pH-responsive release of SRF from the carrier system and we have demonstrated the specific affinity of G-S27LN towards the GPC3-overexpressed HepG2 cancer cells. Anti-microRNA27a significantly increased the protein expression of FOXO1 and PPAR-γ which are crucial components involved in proliferation and apoptosis of tumor cells. Combination of SRF and anti-miRNA27a (G-S27LN) resulted in significantly lower cell viability with a marked increase in the apoptosis cell proportion compared to that of free SRF indicating the synergistic anticancer effect. Animal studies in liver cancer xenograft model demonstrated significant suppression of tumor burden, reduced tumor cell and elevated TUNEL positive apoptosis with no toxicity concerns in animals treated with G-S27LN formulation.

CONCLUSION:

The CSL-based G-S27LN efficiently co-delivered anti-microRNA27a and SRF and therefore represents a promising therapy to treat liver cancer. This study also brings forth a platform strategy for the effective treatment of number of other advanced cancers.

KEYWORDS:

anti-microRNA27a; apoptosis; lipid nanoparticles; liver cancer; sorafenib

PMID:
31396764
DOI:
10.1007/s11095-019-2669-5
[Indexed for MEDLINE]
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12.
N Engl J Med. 2019 Aug 8;381(6):531-542. doi: 10.1056/NEJMoa1715944.

Volanesorsen and Triglyceride Levels in Familial Chylomicronemia Syndrome.

Author information

1
From the Department of Medicine, University of California San Diego, La Jolla (J.L.W., S.T.), and Ionis Pharmaceuticals, Carlsbad (V.J.A., Q.Y., S.G.H., R.S.G., S.T.) - both in California; the Department of Medicine, Université de Montréal and ECOGENE 21, Chicoutimi, QC (D.G.), and the Department of Medicine and Laboratory Medicine, Centre Hospitalier Universitaire de Québec-University Laval, Quebec, QC (J.B.) - both in Canada; the Department of Medicine, Beth Israel Deaconess Medical Center (S.D.F.), and the Department of Medicine, Massachusetts General Hospital (L.H.), Boston, and Akcea Therapeutics, Cambridge (A.D., K.R.W., L.O.) - all in Massachusetts; Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome (M.A.); Academic Medical Center, Department of Vascular Medicine, Amsterdam (E.S.G.S.); the Department of Medicine, Manchester University Hospital NHS Foundation Trust, Manchester, United Kingdom (H.S.); the Department of Internal Medicine, Hospital Universitario Miguel Servet, IIS Aragón, Universidad de Zaragoza, Zaragoza, Spain (F.C.); the Division of Lipidology and Hatter Institute for Cardiovascular Research in Africa, University of Cape Town, Cape Town, South Africa (D.J.B.); and the Department of Endocrinology and Cardiovascular Disease Prevention, Assistance Publique-Hôpitaux de Paris, La Pitié-Salpêtrière Hospital, Institut de Création et d'Animation Numériques, Paris (E.B.).

Abstract

BACKGROUND:

Familial chylomicronemia syndrome is a rare genetic disorder that is caused by loss of lipoprotein lipase activity and characterized by chylomicronemia and recurrent episodes of pancreatitis. There are no effective therapies. In an open-label study of three patients with this syndrome, antisense-mediated inhibition of hepatic APOC3 mRNA with volanesorsen led to decreased plasma apolipoprotein C-III and triglyceride levels.

METHODS:

We conducted a phase 3, double-blind, randomized 52-week trial to evaluate the safety and effectiveness of volanesorsen in 66 patients with familial chylomicronemia syndrome. Patients were randomly assigned, in a 1:1 ratio, to receive volanesorsen or placebo. The primary end point was the percentage change in fasting triglyceride levels from baseline to 3 months.

RESULTS:

Patients receiving volanesorsen had a decrease in mean plasma apolipoprotein C-III levels from baseline of 25.7 mg per deciliter, corresponding to an 84% decrease at 3 months, whereas patients receiving placebo had an increase in mean plasma apolipoprotein C-III levels from baseline of 1.9 mg per deciliter, corresponding to a 6.1% increase (P<0.001). Patients receiving volanesorsen had a 77% decrease in mean triglyceride levels, corresponding to a mean decrease of 1712 mg per deciliter (19.3 mmol per liter) (95% confidence interval [CI], 1330 to 2094 mg per deciliter [15.0 to 23.6 mmol per liter]), whereas patients receiving placebo had an 18% increase in mean triglyceride levels, corresponding to an increase of 92.0 mg per deciliter (1.0 mmol per liter) (95% CI, -301.0 to 486 mg per deciliter [-3.4 to 5.5 mmol per liter]) (P<0.001). At 3 months, 77% of the patients in the volanesorsen group, as compared with 10% of patients in the placebo group, had triglyceride levels of less than 750 mg per deciliter (8.5 mmol per liter). A total of 20 of 33 patients who received volanesorsen had injection-site reactions, whereas none of the patients who received placebo had such reactions. No patients in the placebo group had platelet counts below 100,000 per microliter, whereas 15 of 33 patients in the volanesorsen group had such levels, including 2 who had levels below 25,000 per microliter. No patient had platelet counts below 50,000 per microliter after enhanced platelet-monitoring began.

CONCLUSIONS:

Volanesorsen lowered triglyceride levels to less than 750 mg per deciliter in 77% of patients with familial chylomicronemia syndrome. Thrombocytopenia and injection-site reactions were common adverse events. (Funded by Ionis Pharmaceuticals and Akcea Therapeutics; APPROACH Clinical Trials.gov number, NCT02211209.).

PMID:
31390500
DOI:
10.1056/NEJMoa1715944
[Indexed for MEDLINE]
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13.
J Biomed Nanotechnol. 2019 Sep 1;15(9):1948-1959. doi: 10.1166/jbn.2019.2828.

DNA Nanorobot Delivers Antisense Oligonucleotides Silencing c-Met Gene Expression for Cancer Therapy.

Abstract

Antisense oligonucleotides are considered to be a promising strategy for cancer therapy because of their high specificity and minimal side effects. They can bind specifically to mRNA silencing the expression of target genes. However, ssDNA cannot enter cells in large quantities, which limits its applications. Tetrahedral framework nucleic acids (tFNA) are considered to be optimal nanoscopic drug carriers because of their editability and biocompatibility. Most importantly, they can be modified with functional molecules. The over-expression of c-Met is associated with a wide variety of tumor occurrences, developments, drug resistance and prognoses. Activation of HGF/c-Met signaling pathways can promote cell migration and invasion in cancer. Therefore, blocking the expression of c-Met is a valid technique for cancer therapy. In this study, we used tFNA as carriers to deliver antisense oligonucleotides, which can bind to c-Met mRNA with high specificity and affinity, into cells resulting in the inhibition of c-Met expression for cancer therapy.

PMID:
31387681
DOI:
10.1166/jbn.2019.2828
[Indexed for MEDLINE]
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14.
15.
Medicine (Baltimore). 2019 Aug;98(31):e16694. doi: 10.1097/MD.0000000000016694.

Ultrasound-guided peri-brachial plexus polydeoxyribonucleotide injection for a patient with postherpetic brachial plexopathy: A case report.

Author information

1
Department of Dermatology.
2
Department of Rehabilitation Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital.
3
Department of Neurology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok hospital.
4
Department of Rehabilitation Medicine, Daegu Fatima Hospital, Daegu, South Korea.

Abstract

RATIONALE:

Although most complications of herpes zoster (HZ) are associated with the spread of varicella-zoster virus from the initially involved sensory ganglion, motor nerve impairment, such as limb weakness, is a rare but severe complication that is difficult to treat.

PATIENT CONCERN:

A 73-year-old female presented with sudden left upper limb pain and weakness after HZ.

DIAGNOSIS:

Brachial plexopathy following HZ (postherpetic brachial plexopathy).

INTERVENTION:

Despite alleviation of the vesicles with antiviral treatments, the left upper limb weakness and neuropathic pain did not improve. After obtaining patient's consent, ultrasound-guided polydeoxyribonucleotide (PDRN) injection was performed around the left brachial plexus.

OUTCOMES:

The patient showed marked improvement in left arm pain from numerical rating scale (NRS) 9 to 4, 1 day after PDRN injection. Subsequently, the pain improved to NRS 3, and motor weakness improved to Medical Research Council grade 2 to 4.

LESSONS:

PDRN can be considered a viable substitute for corticosteroid injection in treatment of motor weakness and neuropathic pain after HZ.

PMID:
31374058
PMCID:
PMC6709125
DOI:
10.1097/MD.0000000000016694
[Indexed for MEDLINE]
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16.
Analyst. 2019 Sep 7;144(17):5098-5107. doi: 10.1039/c9an01097a. Epub 2019 Aug 2.

An aptamer-tethered, DNAzyme-embedded molecular beacon for simultaneous detection and regulation of tumor-related genes in living cells.

Author information

1
Xiangya School of Pharmaceutical Sciences, State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410013, China. zhouwenhuyaoji@163.com and Department of Pharmacy, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, China.
2
Xiangya School of Pharmaceutical Sciences, State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410013, China. zhouwenhuyaoji@163.com.

Abstract

Simultaneous detection and regulation of tumor-related genes presents a promising strategy for early diagnosis and treatment of cancer, but achieving this has been a huge challenge for both chemical and biomedical communities. Towards this objective, we have devised a novel aptamer-tethered, DNAzyme-embedded molecular beacon (MB) for multiple functions in cancer cells. In this design, a tumor targeting aptamer was employed to specifically deliver the sensor into cancer cells for target gene detection, and an RNA-cleaving DNAzyme was embedded to realize gene regulation. Both aptamer-tethering and DNAzyme-embedding had little influence on the sensor performance, with a detection limit of ∼2 nM and high specificity. After delivering into tumor cells, our device could monitor the tumor-related genes by producing detectable fluorescence signals, and regulate the gene expression at both mRNA and protein levels as evidenced by the RT-PCR and western blot analyses. This study provides a simple and efficient strategy to rationally combine various functional nucleic acids for multi-functional applications in living cells, which hold great potential for cancer diagnosis and therapy.

PMID:
31373344
DOI:
10.1039/c9an01097a
[Indexed for MEDLINE]
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17.
Chem Pharm Bull (Tokyo). 2019;67(8):877-883. doi: 10.1248/cpb.c19-00337.

Study of the Inducible Cross-Linking Reaction to mRNA and the Effect on the Translation.

Author information

1
Graduate School of Pharmaceutical Sciences, Kyushu University.

Abstract

The 4-vinylpyrimidin-2-one nucleoside (T-vinyl) forms a cross-link with the RNA containing uracil at the complementary site at a high reaction rate. To obtain the stable T-vinyl derivative so that its reactivity is protected until it access to the target site, several derivatives were investigated, and the 2-thiopyridinyl- and 2-thiopyrimidinyl T-vinyl derivatives were determined to be good candidates. The 2-thiopyrimidinyl T-vinyl derivative was found to more efficiently cross-link with mRNA albeit having a better stability than the 2-thiopyridinyl T-vinyl derivative. The investigation using the luciferase (Luc) mRNA, the synthetic mRNA and non-cellular translation system revealed that the translation is terminated at the end of the cross-linked duplex between the mRNA and the oligoribonucleotide (ORN). Thus, the 2-thiopyrimidinyl T-vinyl derivative has successfully demonstrated both a good stability and high efficiency for the cross-linking reaction, and expanded its applicability in biological applications.

KEYWORDS:

4-vinylpyrimidin-2-one nucleoside; cross-link; luciferase mRNA; synthetic mRNA; translation; truncated peptide

PMID:
31366836
DOI:
10.1248/cpb.c19-00337
[Indexed for MEDLINE]
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18.
Arch Virol. 2019 Oct;164(10):2581-2584. doi: 10.1007/s00705-019-04361-3. Epub 2019 Jul 29.

Recombinase polymerase amplification assay for rapid detection of maize chlorotic mottle virus in maize.

Author information

1
College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
2
College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China. zihao8337@syau.edu.cn.
3
College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China. wuyh09@syau.edu.cn.

Abstract

Maize chlorotic mottle virus (MCMV), an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. In this study, recombinase polymerase amplification (RPA), a novel isothermal DNA amplification and detection technique, was developed to detect MCMV in maize crops. A pair of specific primers was designed based on the conserved sequences of the MCMV coat protein region. The RT-RPA assay was carried out as an isothermal reaction at 38 °C that was complete within 30 min, and no cross-reactivity was detected with other viruses infecting maize in China. The limit of detection of the RT-RPA assay was tenfold lower than that of ordinary RT-PCR. Moreover, this method was successfully applied to test field-collected samples. The newly developed RT-RPA assay offers a reliable, sensitive and efficient method for rapid detection of MCMV in maize in equipment-limited diagnostic laboratories and on-site facilities.

PMID:
31359148
DOI:
10.1007/s00705-019-04361-3
[Indexed for MEDLINE]
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19.
Anal Chim Acta. 2019 Oct 31;1078:42-52. doi: 10.1016/j.aca.2019.06.018. Epub 2019 Jun 11.

An electrochemical paper based nano-genosensor modified with reduced graphene oxide-gold nanostructure for determination of glycated hemoglobin in blood.

Author information

1
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran, 1684613114, Iran; Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran, 1684613114, Iran.
2
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology (IUST), Tehran, 1684613114, Iran; Electroanalytical Chemistry Research Center, Iran University of Science and Technology (IUST), Tehran, 1684613114, Iran. Electronic address: ghaffarinejad@iust.ac.ir.
3
Department of Biochemistry, Genetic and Metabolism Research Group, Pasteur Institute of Iran, Tehran, Iran. Electronic address: skandar@pasteur.ac.ir.

Abstract

Hemoglobin A1c (HbA1c) is a standard biomarker to measure long-term average glucose concentration for diagnosis and monitoring of diabetes. Various methods have been reported for measuring HbA1c, however, portable and precise determination is still challenging. Herein, a new highly sensitive electrochemical nanobiosensor is developed for the specific determination of HbA1c. A nanocomposite of reduced graphene oxide (rGO) and gold with hierarchical architecture structure was electrochemically deposited on a cheap and flexible graphite sheet (GS) electrode. The nanocomposite increased the surface area, improved the electron transfer on the electrode surface and augmented the signal. It also provided a suitable substrate for linkage of thiolated DNA aptamer as a bioreceptor on the electrode surface by strong covalent bonding. The quantitative label free detection was carried out by differential pulse voltammetry (DPV) in a phosphate-buffered saline (PBS) solution containing redox probe Fe(CN)63-/4-. The detection is based on insulating the surface in presence of HbA1c and decreasing the current, which is directly related to the HbA1c concentration. The nanobiosensor demonstrated high sensitivity of 269.2 μA. cm-2, wide linear range of 1 nM-13.83 μM with a low detection limit of 1 nM. The biosensor was successfully used for measuring HbA1c in blood real sample. Furthermore, it is promising to use it as a part of a point of care device for low-invasive screening and management of diabetes.

KEYWORDS:

Electrochemical biosensing; Glycated hemoglobin; Nano-genosensor; Paper-based biosensor

PMID:
31358227
DOI:
10.1016/j.aca.2019.06.018
[Indexed for MEDLINE]
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20.
Anal Chim Acta. 2019 Oct 31;1078:24-31. doi: 10.1016/j.aca.2019.05.074. Epub 2019 Jun 1.

A novel electrochemical DNA biosensor for transgenic soybean detection based on triple signal amplification.

Author information

1
College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
2
College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China; Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guangxi, 541004, China. Electronic address: 583776610@qq.com.
3
College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China; Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guangxi, 541004, China. Electronic address: likianping@263.net.

Abstract

A novel electrochemical DNA biosensor was developed and MON89788 of soybean transgenic gene sequence was detected based on a strategy of rolling circle amplification (RCA) and gold nanoparticle cube (AuNPC)-labeled multiple probes. First, the mercapto-modified capture DNA was immobilized on the surface of the Fe3O4@Au magnetic nanoparticles via an Au-S bond, and the capture DNA was opened and complementarily hybridized with the target DNA to form a double-stranded DNA. In the 10 × reaction buffer, Exonuclease III (ExoIII) specifically recognized and sheared the double-stranded DNA to release the target DNA, which led to the next round of reaction. Afterward, AuNP cube-loaded ssDNA (AuNPC/DNA) was added with the rolling circle reaction with the help of Phi29 DNA polymerase and T4 ligase. Finally, [Ru(NH3)6]3+ was attracted directly by the anionic phosphate of ssDNA via electrostatic interaction. The determination was carried out by using chronocoulometry (CC), and the CC signal was recorded. The mass amount of DNA strands extended infinitely on the AuNPs cube and numerous [Ru(NH3)6]3+ were absorbed, thus the detected signal was highly amplified. The corresponding CC signal showed a good linear relationship with the logarithm of the target DNA concentration in the range of 1 × 10-16 to 1 × 10-7 mol L-1, with a detection limit of 4.5 × 10-17 mol L-1. Specific gene sequence of MON89788 in soybean samples was determined, and the recoveries ranged from 97.3% to 102.0%. This sensor is one of the most sensitive sensors for genetic sequence assessment at present. Moreover, it demonstrates good selectivity, stability, and reproducibility.

KEYWORDS:

AuNPs cube; Chronocoulometry; Electrochemical biosensor; Exonuclease III; Rolling circle amplification; Signal amplification

PMID:
31358225
DOI:
10.1016/j.aca.2019.05.074
[Indexed for MEDLINE]
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