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Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12176-81.

Genome sequence of Halobacterium species NRC-1.

Author information

1
Department of Molecular Biotechnology, University of Washington, Seattle, WA 98195, USA. tment of Microbiology, University of Massachusetts, Amherst, MA 01003; Centre for Extremophile Research, Department of Biology and Biochemistry, Univer.

Abstract

We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the Gram-positive Bacillus subtilis and other bacteria. The ease of culturing Halobacterium and the availability of methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this halophile can serve as an excellent model system among the archaea.

PMID:
11016950
PMCID:
PMC17314
DOI:
10.1073/pnas.190337797
[Indexed for MEDLINE]
Free PMC Article

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