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BMC Genomics. 2019 Jul 15;20(1):583. doi: 10.1186/s12864-019-5955-z.

Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae).

Author information

1
Department of Ecology and Evolution Biophore, University of Lausanne, Lausanne, Switzerland.
2
Present Address: Swiss Federal Institute of Technology (EPFL), 1015, Lausanne, Switzerland.
3
Swiss Institute of Bioinformatics, Lausanne, Switzerland.
4
Present Address: Department of Biomedicine, University of Basel, 4031, Basel, Switzerland.
5
Present Address: Department of Environmental Sciences, Policy and Management, University of California, Berkeley, CA, 94720, USA.
6
Swiss Centre for Applied Ecotoxicology Eawag-EPFL, Dübendorf, Switzerland.
7
Aquatic Ecology and Toxicology Group Center of Organismic Studies, University of Heidelberg, Heidelberg, Germany.
8
Department of Ecology and Evolution Biophore, University of Lausanne, Lausanne, Switzerland. claus.wedekind@unil.ch.

Abstract

The synthetic 17α-ethinylestradiol (EE2) is a common estrogenic pollutant that has been suspected to affect the demography of river-dwelling salmonids. One possibility is that exposure to EE2 tips the balance during initial steps of sex differentiation, so that male genotypes show female-specific gene expression and gonad formation. Here we study EE2 effects on gene expression around the onset of sex differentiation in a population of European grayling (Thymallus thymallus) that suffers from sex ratio distortions. We exposed singly-raised embryos to one dose of 1 ng/L EE2, studied gene expression 10 days before hatching, at the day of hatching, and around the end of the yolk-sac stage, and related it to genetic sex (sdY genotype). We found that exposure to EE2 affects expression of a large number of genes, especially around hatching. These effects were strongly sex-dependent. We then raised fish for several months after hatching and found no evidence of sex reversal in the EE2-exposed fish. We conclude that ecologically relevant (i.e. low) levels of EE2 pollution do not cause sex reversal by simply tipping the balance at early stages of sex differentiation, but that they interfere with sex-specific gene expression.

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