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J Glob Antimicrob Resist. 2019 Dec;19:255-261. doi: 10.1016/j.jgar.2019.05.005. Epub 2019 May 14.

Efficacy of newly isolated and highly potent bacteriophages in a mouse model of extensively drug-resistant Acinetobacter baumannii bacteraemia.

Author information

1
The Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilissi, Georgia.
2
Department of Fundamental Microbiology, University of Lausanne, Switzerland.
3
Department of Fundamental Microbiology, University of Lausanne, Switzerland; Department of Biochemistry, Microbiology and Bio-Informatic, University Laval, Québec, Canada.
4
Department of Intensive Medicine, Inselspital, Bern, Switzerland.
5
Department of Fundamental Microbiology, University of Lausanne, Switzerland. Electronic address: gregory.resch@unil.ch.

Abstract

OBJECTIVES:

Bacteraemia can be caused by Acinetobacter baumannii (A. baumannii), with clinical manifestations ranging from transient bacteraemia to septic shock. Extensively drug-resistant A. baumannii (XDRAB) strains producing the New Delhi metallo-ß-lactamase, which confers resistance to all ß-lactams including carbapenems, have emerged. Infected patients suffer increased mortality, morbidity and length of hospitalisation. The lack of new antimicrobials has led to a renewed interest in phage therapy, the so-called forgotten cure. Accordingly, we tested new lytic bacteriophages in a Galleria mellonella and a mouse model of XDRAB-induced bacteraemia.

METHODS:

Galleria mellonella were challenged with 5.105 CFU of the XDRAB strain FER. Phages vB_AbaM_3054 and vB_AbaM_3090 were administrated alone or in combination 30min after bacterial challenge. Saline and imipenem were injected as controls. Mice were intraperitoneally (i.p.) challenged with 6.107 CFU of A. baumannii FER. vB_AbaM_3054 and vB_AbaM_3090 were administrated i.p. alone or in combination 2h after bacterial challenge. Saline and imipenem were injected as controls. Larvae and mice survival were followed for 7 days and compared with Log-Rank (Mantel-Cox) and Gehan-Breslow-Wilcoxon tests.

RESULTS:

Phage-based treatments showed high efficacy in larvae (ca. 100% survival at 80h) and mice (ca. 100% survival at day 7) compared with the untreated controls (0% survival at 48h and 24h in larvae and mice, respectively).

CONCLUSIONS:

The present data reporting efficacy of phage therapy in a mouse model of bacteraemia support the development of phage-based drugs to manage infection due to multi-drug resistant A. baumannii and particularly XDRAB.

KEYWORDS:

Bacteraemia; Bacteriophage; Extensively drug-resistant A. baumannii (XDRAB); Multidrug-resistant A. baumannii (MDRAB); Phage therapy; Sepsis

PMID:
31100499
DOI:
10.1016/j.jgar.2019.05.005
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