Format

Send to

Choose Destination
PLoS One. 2016 Nov 3;11(11):e0165850. doi: 10.1371/journal.pone.0165850. eCollection 2016.

The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.

Author information

1
Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
2
Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland.
3
SIB Swiss Institute for Bioinformatics, Lausanne, Switzerland.
4
Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland.
5
Master in Molecular Life Sciences, University of Lausanne, Lausanne, Switzerland.
6
Bioinformatics and Systems Biology, Justus-Liebig-University, Gießen, Germany.
7
Lausanne Genomic Technologies Facility, Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland.

Abstract

The natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (BTEX) may be accelerated by inoculation of specific biodegraders (bioaugmentation). Bioaugmentation mainly involves introducing bacteria that deploy their metabolic properties and adaptation potential to survive and propagate in the contaminated environment by degrading the pollutant. In order to better understand the adaptive response of cells during a transition to contaminated material, we analyzed here the genome and short-term (1 h) changes in genome-wide gene expression of the BTEX-degrading bacterium Pseudomonas veronii 1YdBTEX2 in non-sterile soil and liquid medium, both in presence or absence of toluene. We obtained a gapless genome sequence of P. veronii 1YdBTEX2 covering three individual replicons with a total size of 8 Mb, two of which are largely unrelated to current known bacterial replicons. One-hour exposure to toluene, both in soil and liquid, triggered massive transcription (up to 208-fold induction) of multiple gene clusters, such as toluene degradation pathway(s), chemotaxis and toluene efflux pumps. This clearly underlines their key role in the adaptive response to toluene. In comparison to liquid medium, cells in soil drastically changed expression of genes involved in membrane functioning (e.g., lipid composition, lipid metabolism, cell fatty acid synthesis), osmotic stress response (e.g., polyamine or trehalose synthesis, uptake of potassium) and putrescine metabolism, highlighting the immediate response mechanisms of P. veronii 1YdBTEX2 for successful establishment in polluted soil.

PMID:
27812150
PMCID:
PMC5094676
DOI:
10.1371/journal.pone.0165850
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center