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Int J Syst Evol Microbiol. 2020 Feb 11. doi: 10.1099/ijsem.0.003909. [Epub ahead of print]

Brevilactibacter flavus gen. nov., sp. nov., a novel bacterium of the family Propionibacteriaceae isolated from raw milk and dairy products and reclassification of Propioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov.

Author information

1
Bavarian Health and Food Safety Authority, Veterinärstraße 2, 85764 Oberschleißheim, Germany.
2
Chair for Microbial Ecology, ZIEL - Institute for Food & Health, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany.
3
Present address: Tetra Holdings GmbH, Untere Waldplätze 27, 70569 Stuttgart, Germany.
4
Milchprüfring Baden-Württemberg e.V., 73230 Kirchheim unter Teck, Germany.
5
Bavarian Center for Biomolecular Mass Spectrometry, Technische Universität München, 85354 Freising, Germany.
6
Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München, 85354 Freising, Germany.
7
Present address: Eurofins BioPharma Product Testing Munich GmbH, Behringstr. 6 / 8, 82152 Planegg, Germany.
8
Intstitute of Microbiology, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria.

Abstract

Eight facultatively anaerobic rod-shaped bacteria were isolated from raw milk and two other dairy products. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates are placed in a distinct lineage within the family Propionibacteriaceae with Propioniciclava sinopodophylli and Propioniciclava tarda as the closest relatives (94.6 and 93.5 % similarity, respectively). The cell-wall peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid and was of the A1γ type (meso-DAP-direct). The major cellular fatty acid was anteiso-C15 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidyglycerol and three unidentified glycolipids. The quinone system contained predominantly menaquinone MK-9(H4). The G+C content of the genomic DNA of strain VG341T was 67.7 mol%. The whole-cell sugar pattern contained ribose, rhamnose, arabinose and galactose. On the basis of phenotypic and genetic data, eight strains (VG341T, WS4684, WS4769, WS 4882, WS4883, WS4901, WS4902 and WS4904) are proposed to be classified as members of a novel species in a new genus of the family Propionibacteriaceae, for which the name Brevilactibacter flavus gen. nov., sp. nov. is proposed. The type strain is VG341T (=WS4900T=DSM 100885T=LMG 29089T) and seven additional strains are WS4684, WS4769, WS4882, WS4883, WS4901, WS4902 and WS4904. Furthermore, we propose the reclassification of P. sinopodophylli as Brevilactibacter sinopodophylli comb. nov.

KEYWORDS:

Brevilactibacter flavus; Propionibacteriaceae; raw milk

PMID:
32043954
DOI:
10.1099/ijsem.0.003909

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