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Vector Borne Zoonotic Dis. 2019 Aug 13. doi: 10.1089/vbz.2019.2444. [Epub ahead of print]

Leptospira Seroprevalence Detection and Rabies Virus Absence in an Urban Raccoon (Procyon lotor) Population in a Highly Populated Area, Costa Rica.

Author information

1
1Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, Austria.
2
2Tropical Diseases Research Program, School of Veterinary Medicine, National University, Heredia, Costa Rica.
3
3National Animal Health Service (SENASA), Ministry of Agriculture and Livestock (MAG), Barreal, Costa Rica.
4
4Institute of Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.
5
5Pathology Department, School of Veterinary Medicine, National University, Heredia, Costa Rica.
6
6Wildlife Conservation Society, Wildlife Health Program, Bronx, New York.

Abstract

Leptospirosis and rabies are zoonotic diseases of public health importance and endemic diseases in tropical countries such as Costa Rica. Peridomestic wild animals such as raccoons (Procyon lotor) have been implicated as competent hosts of Leptospira spirochetes and rabies virus. This study focused on understanding the role of urban raccoons in the dynamics of leptospirosis and rabies in a tropical environment. A total of 97 specimens of the common raccoon were captured within the Greater Metropolitan Area of Costa Rica; 32.6% (31/95) of raccoons presented evidence of antibodies (> 1: 100) against Leptospira sp. Attempts to cultivate Leptospira failed, but 19 serovars were identified, which are also responsible for causing disease in humans in Costa Rica. Detected titers ranged from 1: 100 to 1: 6400. Lymphoid hyperplasia in kidneys and spirochetes were demonstrated in 3 of 20 necropsied cases (15%). Twenty brain samples were analyzed by hematoxylin and eosin stain for evidence of encephalitis and Negri body detection and simultaneously frozen brain material was employed to perform a rapid immunoassay test for rabies antigen. All tested samples were negative. This study is the first report of Leptospira seroprevalence in raccoons that cohabit urban areas in Costa Rica. We also highlight the importance of the raccoon as one of their natural competent host and sentinel animals within highly populated urban environments in tropical cities.

KEYWORDS:

; leptospirosis; public health; rabies virus; zoonosis

PMID:
31407956
DOI:
10.1089/vbz.2019.2444

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