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J Biomater Appl. 2016 Jul;31(1):45-54. doi: 10.1177/0885328216630912. Epub 2016 Feb 10.

In vivo quantification of gentamicin released from an implant coating.

Author information

1
Berlin-Brandenburg Center for Regenerative Therapies and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Berlin, Germany.
2
Excellence Cluster Cardio-Pulmonary System, Giessen, Germany.
3
Synthes GmbH, Oberdorf, Switzerland.
4
Chirurgische Klinik Dr. Rinecker, München, Germany.
5
Zentrum für Orthopädie, Unfallchirurgie und Paraplegiologie, Universitätsklinikum Heidelberg, Heidelberg, Germany.
6
Berlin-Brandenburg Center for Regenerative Therapies and Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Berlin, Germany britt.wildemann@charite.de.

Abstract

Drug-releasing implants are gaining increasing interest. The present study reports a detailed physicochemical analysis of a polymeric coating based on poly(D,L-lactide) and the incorporated gentamicin combined with an in vitro and in vivo study of the gentamicin release. Differential scanning calorimeter, Fourier transform infrared spectroscopy, gel permeation chromatography and high-performance liquid chromatography showed no effect of the gamma sterilisation on the coating components or an interaction of the polymer and the gentamicin. Microbiological analysis revealed an inhibition of bacterial growth on the implant surface. For the in vivo study, gentamicin-coated wires were implanted into the tibiae of rats and harvested at different time points up to day 42. To monitor the release in vivo, gentamicin was quantified in serum, bone, endosteum, kidney, and on the explanted wires. Gentamicin was detectable over a time period of 42 days in the endosteum, up to seven days in the kidney, up to 4 h in the bone and at the end of the experiment on one of eight wires. The locally released gentamicin caused no histological changes of the kidney. Microbiologically active concentrations of released gentamicin were found in the endosteum up to 4 h after implantation. The combination of different methods supports the individual results, where quantification is complemented by visualisation or antimicrobial activity. This work demonstrates that the coating procedure results in no substantial alteration of the incorporated drug and that the in vitro burst release occurs also in vivo.

KEYWORDS:

Gentamicin coating; drug release; immunohistochemistry; in vitro/in vivo; microbiology; physicochemical properties

PMID:
26865659
DOI:
10.1177/0885328216630912
[Indexed for MEDLINE]

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