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Vet Microbiol. 2017 Feb;200:124-129. doi: 10.1016/j.vetmic.2015.11.030. Epub 2015 Nov 25.

Prevalence of carbapenemase producing Enterobacteriaceae isolated from German pig-fattening farms during the years 2011-2013.

Author information

1
Freie Universitaet Berlin, Institute for Animal Hygiene and Environmental Health, Robert-von-Ostertag-Straße 7-13, 14163 Berlin, Germany. Electronic address: tierhygiene@vetmed.fu-berlin.de.
2
Freie Universitaet Berlin, Institute for Animal Hygiene and Environmental Health, Robert-von-Ostertag-Straße 7-13, 14163 Berlin, Germany.
3
University of Veterinary Medicine Hannover, Institute for Biometrics, Epidemiology and Information Processing, Buenteweg 2, 30559 Hannover, Germany.
4
Federal Institute for Risk Assessment, Department: Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany.

Abstract

Since recently Enterobacteriaceae carrying blaVIM-1 genes have been isolated in German animal husbandries, the monitoring of carbapenemase producing Enterobacteriaceae (CPE) in livestock became a major topic within the European Union. Nevertheless, due to missing surveillance studies the worldwide situation in livestock and livestock associated surroundings might still be underestimated. The here described study provides an overview of the CPE-prevalence in German pig-fattening farms during the years 2011-2013 (period when previously described blaVIM-1 findings occurred on pig-fattening farms (Efsa, 2011; Fischer et al., 2012, 2013a)). Therefore, a collection of 238 bacterial anacultures derived from pooled faeces and boot swab samples, collected in a cross-sectional study including 58 pig-fattening farms throughout Germany, were investigated. The bacteria were selected on MacConkey agar plates containing 0.125μg/ml meropenem. Enterobacteriaceae which were able to grow on these plates were further investigated for the presence of carbapenemase genes. Out of eight CPE-suspicious strains, two Escherichia (E.) coli strains-deriving from the same farm-contained the carbapenemase gene blaVIM-1. For the remaining six Enterobacteriaceae it seems to be likely that they possess other resistance mechanisms, leading to reduced carbapenem susceptibility. Based on the obtained results, the overall CPE prevalence for German pig-fattening farms, sampled during the years 2011-2013 was 1.7%; 95% CI: 0-10. However, as it is of great importance to prevent a further spread of these bacteria between farms and livestock populations as well as their introduction into the food chain, an understanding of their routes of introduction and spread in combination with intensified monitoring programs are considered necessary.

KEYWORDS:

Beta-lactam antibiotics; CPE; Livestock; Multidrug resistance

PMID:
26654218
DOI:
10.1016/j.vetmic.2015.11.030
[Indexed for MEDLINE]

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