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Biomed Res Int. 2015;2015:628158. doi: 10.1155/2015/628158. Epub 2015 Oct 1.

Cross-omics comparison of stress responses in mesothelial cells exposed to heat- versus filter-sterilized peritoneal dialysis fluids.

Author information

1
Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, Austria ; Zytoprotec GmbH, 1090 Vienna, Austria.
2
Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, Austria ; Department of Nephrology and Medical Intensive Care, Campus Virchow-Klinikum, Charité Universitätsmedizin Berlin, 13353 Berlin, Germany.
3
Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, Austria.
4
Zytoprotec GmbH, 1090 Vienna, Austria.
5
Department of Nephrology and Medical Intensive Care, Campus Virchow-Klinikum, Charité Universitätsmedizin Berlin, 13353 Berlin, Germany.

Abstract

Recent research suggests that cytoprotective responses, such as expression of heat-shock proteins, might be inadequately induced in mesothelial cells by heat-sterilized peritoneal dialysis (PD) fluids. This study compares transcriptome data and multiple protein expression profiles for providing new insight into regulatory mechanisms. Two-dimensional difference gel electrophoresis (2D-DIGE) based proteomics and topic defined gene expression microarray-based transcriptomics techniques were used to evaluate stress responses in human omental peritoneal mesothelial cells in response to heat- or filter-sterilized PD fluids. Data from selected heat-shock proteins were validated by 2D western-blot analysis. Comparison of proteomics and transcriptomics data discriminated differentially regulated protein abundance into groups depending on correlating or noncorrelating transcripts. Inadequate abundance of several heat-shock proteins following exposure to heat-sterilized PD fluids is not reflected on the mRNA level indicating interference beyond transcriptional regulation. For the first time, this study describes evidence for posttranscriptional inadequacy of heat-shock protein expression by heat-sterilized PD fluids as a novel cytotoxic property. Cross-omics technologies introduce a novel way of understanding PDF bioincompatibility and searching for new interventions to reestablish adequate cytoprotective responses.

PMID:
26495307
PMCID:
PMC4606138
DOI:
10.1155/2015/628158
[Indexed for MEDLINE]
Free PMC Article

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