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J Vis Exp. 2014 Sep 30;(91):e51706. doi: 10.3791/51706.

Whole-cell patch-clamp recordings from morphologically- and neurochemically-identified hippocampal interneurons.

Author information

1
Institute of Integrative Neuroanatomy, NeuroCure Cluster of Excellence, Charité Universitätmedizin.
2
Institute of Integrative Neuroanatomy, NeuroCure Cluster of Excellence, Charité Universitätmedizin; Imre.Vida@charite.de.

Abstract

GABAergic inhibitory interneurons play a central role within neuronal circuits of the brain. Interneurons comprise a small subset of the neuronal population (10-20%), but show a high level of physiological, morphological, and neurochemical heterogeneity, reflecting their diverse functions. Therefore, investigation of interneurons provides important insights into the organization principles and function of neuronal circuits. This, however, requires an integrated physiological and neuroanatomical approach for the selection and identification of individual interneuron types. Whole-cell patch-clamp recording from acute brain slices of transgenic animals, expressing fluorescent proteins under the promoters of interneuron-specific markers, provides an efficient method to target and electrophysiologically characterize intrinsic and synaptic properties of specific interneuron types. Combined with intracellular dye labeling, this approach can be extended with post-hoc morphological and immunocytochemical analysis, enabling systematic identification of recorded neurons. These methods can be tailored to suit a broad range of scientific questions regarding functional properties of diverse types of cortical neurons.

PMID:
25350149
PMCID:
PMC4672954
DOI:
10.3791/51706
[Indexed for MEDLINE]
Free PMC Article

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