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Parasitol Int. 2007 Mar;56(1):65-9. Epub 2006 Dec 29.

Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood.

Author information

1
Department of Pathobiology, Mahidol University, Bangkok, Thailand. scrud@mahidol.ac.th

Abstract

Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.

PMID:
17254835
DOI:
10.1016/j.parint.2006.12.005
[Indexed for MEDLINE]

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