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Appl Microbiol. 1972 May;23(5):923-30.

Mass purification of nucleopolyhedrosis virus inclusion bodies in the K-series centrifuge.

Abstract

Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a continuous-sample-flow-with-isopycnic-banding centrifuge in quantities up to 6 x 10(13) polyhedral inclusion bodies per day. Continuous-flow methods for S-rho type purification have been evolved to deal with mass isolation of bioparticles.

PMID:
5031562
PMCID:
PMC380472
[Indexed for MEDLINE]
Free PMC Article

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