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ACS Chem Biol. 2017 Jul 21;12(7):1867-1873. doi: 10.1021/acschembio.7b00337. Epub 2017 Jun 6.

Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores.

Author information

1
Institute of Bioorganic Chemistry, Russian Academy of Sciences , Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
2
Pirogov Russian National Research Medical University , Ostrovitianov 1, 117997 Moscow, Russia.
3
Centre for Analysis and Synthesis, Department of Chemistry, Lund University , 22100 Lund, Sweden.
4
Moscow Institute of Physics and Technology , Institutsky per., 9, 141701 Dolgoprudny, Russia.

Abstract

Rapid development of new microscopy techniques exposed the need for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores. We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-based chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.

PMID:
28525263
DOI:
10.1021/acschembio.7b00337
[Indexed for MEDLINE]

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