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Antimicrob Agents Chemother. 2017 Apr 24;61(5). pii: e02550-16. doi: 10.1128/AAC.02550-16. Print 2017 May.

Molecular Epidemiology of Colistin-Resistant, Carbapenemase-Producing Klebsiella pneumoniae in Serbia from 2013 to 2016.

Author information

1
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
2
Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.
3
Institute for Pulmonary Diseases of Vojvodina, Sremska Kamenica, Serbia.
4
Institute for Laboratory Diagnostics Konzilijum, Belgrade, Serbia.
5
Department of Clinical Microbiology, Mother and Child Health Care Institute of Serbia Dr. Vukan Cupic, Belgrade, Serbia.
6
Institute of Public Health of Vojvodina, Novi Sad, Serbia.
7
Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
8
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia bjovcic@bio.bg.ac.rs.
9
Faculty of Biology, University of Belgrade, Belgrade, Serbia.

Abstract

Twenty-seven colistin-resistant, carbapenemase-producing Klebsiella pneumoniae isolates were identified from hospitals in Serbia. All isolates were blaCTX-M-15 positive; ST101, ST888, ST437, ST336, and ST307 were blaOXA-48 positive; and ST340 was blaNDM-1 positive. ST307 had an insertion, and ST336 had a premature stop codon in the mgrB gene. Amino acid substitutions were detected in PmrAB of isolates ST101, ST888, ST336, and ST307. The mcr-1 and mcr-2 were not detected. An increase in phoP, phoQ, and pmrK gene transcription was detected for all sequence types.

KEYWORDS:

Klebsiella pneumoniae; carbapenem resistance; colistin resistance; molecular epidemiology

PMID:
28242665
PMCID:
PMC5404574
DOI:
10.1128/AAC.02550-16
[Indexed for MEDLINE]
Free PMC Article

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