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Chem Commun (Camb). 2017 Jan 10;53(5):949-951. doi: 10.1039/c6cc09200d.

Intrinsic blinking of red fluorescent proteins for super-resolution microscopy.

Author information

1
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
2
Institute of Applied Physics, Nizhny Novgorod, Russia.
3
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
4
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
5
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru and Pirogov Russian National Research Medical University, Moscow, Russia.

Abstract

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

PMID:
28044165
DOI:
10.1039/c6cc09200d
[Indexed for MEDLINE]

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