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J Microbiol Methods. 2017 Jan;132:21-26. doi: 10.1016/j.mimet.2016.11.008. Epub 2016 Nov 9.

Green fluorescent protein labeling of food pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis.

Author information

1
AIT Austrian Institute of Technology GmbH, Bioresources Unit, Konrad Lorenz Strasse 24, A-3430 Tulln, Austria. Electronic address: Eva.Gensberger@ait.ac.at.
2
AIT Austrian Institute of Technology GmbH, Bioresources Unit, Konrad Lorenz Strasse 24, A-3430 Tulln, Austria.

Abstract

Labeling of bacteria with marker genes, such as green fluorescent protein, is a useful and practicable tool for tracking and enumerating bacterial cells in a complex environment e.g. discrimination from the indigenous background population. In this study, novel TurboGFP prokaryotic expression vector was utilized for labeling of Yersinia species. Y. enterocolitica biovar 1A, biovar 2, biovar 4 and Y. pseudotuberculosis were successfully transformed with the vector and expressed bright green fluorescence that was even detectable visually by eye. No adverse effects were observed in growth behavior of the labeled strains compared to wild type (parental) strains and vector maintenance for longer time periods could be achieved for Y. enterocolitica biovar 1A, Y. enterocolitica biovar 2 and Y. pseudotuberculosis.

PMID:
27838541
DOI:
10.1016/j.mimet.2016.11.008
[Indexed for MEDLINE]

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