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Ann Clin Transl Neurol. 2016 Aug 28;3(10):812-818. eCollection 2016 Oct.

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies.

Author information

1
The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
2
The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom; Centre for Clinical Brain Sciences Western General Hospital Edinburgh EH4 2XU United Kingdom.
3
Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
4
Oxford Project to Investigate Memory and Ageing (OPTIMA) Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
5
Oxford Parkinson's Disease Centre Department of Physiology Anatomy and Genetics University of Oxford Le Gros Clark Building, South Parks Road Oxford OX1 3QX United Kingdom.

Abstract

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

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