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Dokl Biochem Biophys. 2015;465:410-2. doi: 10.1134/S1607672915060174. Epub 2016 Jan 5.

Far-red fluorescent cell line for preclinical study of HER2-targeted agents.

Author information

1
Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia. irin-b@mail.ru.
2
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. irin-b@mail.ru.
3
Lobachevsky Nizhni Novgorod State University, Nizhni Novgorod, 603600, Russia.
4
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.

Abstract

We have created novel HER2-overexpressing human ovarian adenocarcinoma cell line stably transfected with far-red fluorescent protein TurboFP635. Growth characteristics, adhesion capacity and morphology of the cells do not differ from that of parental cell line. The obtained cell line (SKOV-kat) was confirmed to have high level of expression of the tumor marker HER2 comparable to that of initial cell line SKOV-3. The SKOV-kat cell line is designed for further establishment of experimental fluorescent models of HER2-overexpressing human ovarian cancer that make it possible to optimize and reduce the cost of preclinical study of agents for treatment of this disease.

PMID:
26728737
DOI:
10.1134/S1607672915060174
[Indexed for MEDLINE]

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