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Sci Rep. 2015 Nov 20;5:16929. doi: 10.1038/srep16929.

Spatially-resolved intracellular sensing of hydrogen peroxide in living cells.

Author information

1
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
2
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
3
Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Abstract

Understanding intracellular redox chemistry requires new tools for the site-specific visualization of intracellular oxidation. We have developed a spatially-resolved intracellular sensor of hydrogen peroxide, HyPer-Tau, for time-resolved imaging in live cells. This sensor consists of a hydrogen peroxide-sensing protein tethered to microtubules. We demonstrate the use of the HyPer-Tau sensor for three applications; dose-dependent response of human cells to exogenous hydrogen peroxide, a model immune response of mouse macrophages to stimulation by bacterial toxin, and a spatially-resolved response to localized delivery of hydrogen peroxide. These results demonstrate that HyPer-Tau can be used as an effective tool for tracking changes in spatially localized intracellular hydrogen peroxide and for future applications in redox signaling.

PMID:
26585385
PMCID:
PMC4653655
DOI:
10.1038/srep16929
[Indexed for MEDLINE]
Free PMC Article

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