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Oncotarget. 2015 Oct 13;6(31):30919-28. doi: 10.18632/oncotarget.5130.

A novel far-red fluorescent xenograft model of ovarian carcinoma for preclinical evaluation of HER2-targeted immunotoxins.

Author information

1
Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia.
2
Institute of Bioorganic Chemistry of The Russian Academy of Sciences, Moscow 117997, Russia.
3
National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
4
Institute of Applied Physics of The Russian Academy of Sciences, Nizhny Novgorod, 603950 Russia.

Abstract

We have created a novel fluorescent model of a human ovarian carcinoma xenograft overexpressing receptor HER2, a promising molecular target of solid tumors. The model is based on a newly generated SKOV-kat cell line stably expressing far-red fluorescent protein Katushka. Katushka is most suitable for the in vivo imaging due to an optimal combination of high brightness and emission in the "window of tissue transparency". The relevance of the fluorescent model for the in vivo monitoring of tumor growth and response to treatment was demonstrated using a newly created HER2-targeted recombinant immunotoxin based on the 4D5scFv antibody and a fragment of the Pseudomonas exotoxin A.

KEYWORDS:

HER2; far-red fluorescent protein; immunotoxin; ovarian carcinoma; xenograft tumor model

PMID:
26436696
PMCID:
PMC4741577
DOI:
10.18632/oncotarget.5130
[Indexed for MEDLINE]
Free PMC Article

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