Format

Send to

Choose Destination
PLoS One. 2013 Oct 18;8(10):e76871. doi: 10.1371/journal.pone.0076871. eCollection 2013.

Generation of BAC transgenic epithelial organoids.

Author information

1
Hubrecht Institute, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.

Abstract

Under previously developed culture conditions, mouse and human intestinal epithelia can be cultured and expanded over long periods. These so-called organoids recapitulate the three-dimensional architecture of the gut epithelium, and consist of all major intestinal cell types. One key advantage of these ex vivo cultures is their accessibility to live imaging. So far the establishment of transgenic fluorescent reporter organoids has required the generation of transgenic mice, a laborious and time-consuming process, which cannot be extended to human cultures. Here we present a transfection protocol that enables the generation of recombinant mouse and human reporter organoids using BAC (bacterial artificial chromosome) technology.

PMID:
24204693
PMCID:
PMC3800075
DOI:
10.1371/journal.pone.0076871
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center