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J Clin Virol. 2013 Mar;56(3):250-4. doi: 10.1016/j.jcv.2012.11.020. Epub 2012 Dec 27.

Human rhinoviruses including group C are common in stool samples of young Finnish children.

Author information

1
University of Tampere, School of Medicine, Tampere, Finland. hanna.honkanen@uta.fi

Abstract

BACKGROUND:

Human rhinoviruses (HRVs) are common causes of viral respiratory infections. They have been widely studied in respiratory samples in hospital patient series but only a few studies have been performed to assess their occurrence in other sample types and their circulation in healthy children background population.

OBJECTIVES:

To analyze the frequency of HRVs in the background population in Finland by screening HRV RNA from stool samples longitudinally collected in a cohort of young children.

STUDY DESIGN:

Altogether 4184 stool samples were collected regularly from a cohort of children who were observed from birth. Samples were screened for the presence of RNA of HRVs using RT-PCR. HRV specific sequences were identified by sequencing the VP1 or VP4/VP2 coding region. Virus isolation was performed using four different cell lines and the result was confirmed by real time PCR.

RESULTS:

A total of 9% of the stool samples were positive for HRV RNA. Sequence analysis indicated that the most prevalent species was HRV-A, and the most prevalent serotype was HRV61. HRV-B and HRV-C species were also detected. One of the six tested rhinovirus positive samples retained its infectivity and was able to grow in RD and GMK cells.

CONCLUSIONS:

Our study shows that HRVs are frequently detected in the stool samples from the population of young children. We also show that HRV-C, which can cause severe illnesses in children, is commonly circulating in young children in Finland.

PMID:
23273491
DOI:
10.1016/j.jcv.2012.11.020
[Indexed for MEDLINE]

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