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J Histochem Cytochem. 2012 Feb;60(2):152-8. doi: 10.1369/0022155411429704. Epub 2011 Nov 22.

A simple method for the immunocytochemical detection of proteins inside nuclear structures that are inaccessible to specific antibodies.

Author information

1
AN Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.

Abstract

It has been demonstrated elsewhere that a high concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies. In this study, the authors found that a short proteinase treatment allowed for the detection of antigens in the nucleoli. The described approach is compatible with the simultaneous observation of proteins fused to fluorescent tags and with preembedding electron microscopy. It appears that the described method can be useful in situations when the proper recognition of antigens by specific antibodies is disturbed by a high density of cellular structures or a high concentration of antigens inside these structures.

PMID:
22114257
PMCID:
PMC3351117
DOI:
10.1369/0022155411429704
[Indexed for MEDLINE]
Free PMC Article

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