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Plant Signal Behav. 2011 Dec;6(12):1914-7.

Qualitative difference between "bulb" membranes and other vacuolar membranes.

Author information

1
Molecular Membrane Biology Laboratory, RIKEN Advanced Science Institute, Wako, Japan. chiezo@riken.jp

Abstract

"Bulb" is a mobile and complex structure appearing in vacuolar membrane of plant cell. We recently reported new fluorescent marker lines for bulbs and bulb-less mutants. We tried multicolor visualization of vacuolar membrane to show distinct segregation of bulb-positive protein (γTIP or AtVAM3) and bulb-negative protein (AtRab75). Unexpectedly, GFP-AtRab75 resulted to localize in bulb under the condition of co-expression with TagRFP-AtVAM3. The signal intensities of GFP-AtRab75 and TagRFP-AtVAM3 were quantified and compared. The result indicates that TagRFP-AtVAM3 is concentrated in bulb than GFP-AtRab75.

PMID:
22105033
PMCID:
PMC3337177
[Indexed for MEDLINE]
Free PMC Article

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