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Biotechnol Lett. 2009 Feb;31(2):251-7. doi: 10.1007/s10529-008-9850-y. Epub 2008 Sep 23.

Thermolabile duplex-specific nuclease.

Author information

1
Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia. evanika@yandex.ru

Abstract

Using random mutagenesis of the gene encoding duplex-specific nuclease from the king crab we found a new mutant that retained all properties of the wild-type protein, but exhibited a much lower thermal stability. This enzyme, denoted thermolabile duplex-specific nuclease (DSN-TL), exhibits high processivity and selective cleavage of dsDNA. The inactivation temperature for DSN-TL is 15-20 degrees C lower than that of the widely used DNase I and shrimp nuclease, and its catalytic activity is more than 10 times higher. Moreover, DSN-TL is resistant to proteinase K treatment. These properties make DSN-TL very useful for removing genomic DNA from RNA samples intended for quantitative RT-PCR.

PMID:
18810329
DOI:
10.1007/s10529-008-9850-y
[Indexed for MEDLINE]

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