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J Proteome Res. 2005 Jul-Aug;4(4):1451-6.

Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).

Author information

1
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.

Abstract

Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.

PMID:
16083299
DOI:
10.1021/pr050053l
[Indexed for MEDLINE]

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