Format

Send to

Choose Destination
Mol Cell Probes. 2005 Feb;19(1):1-8.

Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans.

Author information

1
State Research Center of Virology and Biotechnology Vector, Koltsovo Novosibirsk Oblast, Koltsovo 630559, Russia. snshchel@vector.nsc.ru

Abstract

A method for one-stage rapid identification of four orthopoxvirus species pathogenic to humans based on multiplex polymerase chain reaction (MPCR) was developed. Five pairs of oligonucleotide primers--one, genus-specific; and the rest, species-specific for variola, monkeypox, cowpox, and vaccinia viruses, respectively--were used concurrently for MPCR assay of orthopoxvirus DNAs. Specificity and sensitivity of the method developed were evaluated using DNAs of 57 orthopoxvirus strains, including the DNAs isolated from human case clinical materials.

PMID:
15652214
DOI:
10.1016/j.mcp.2004.07.004
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center