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Antimicrob Agents Chemother. 2003 Jul;47(7):2362-5.

In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin.

Author information

1
Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.

Abstract

Mutants of attenuated Bacillus anthracis with high-level ciprofloxacin resistance were isolated using a three-step in vitro selection. Ciprofloxacin MICs were 0.5 micro g/ml for first-step mutants, which had one of two gyrA quinolone resistance-determining region (QRDR) mutations. Ciprofloxacin MICs were 8 and 16 microg/ml for second-step mutants, which had one of three parC QRDR mutations. Ciprofloxacin MICs for third-step mutants were 32 and 64 microg/ml. Mutants for which MICs were 64 microg/ml had one of two additional mutations within the gyrA QRDR or one of two mutations within the gyrB QRDR. Mutants for which MICs were 32 microg/ml had no additional target modifications but showed evidence of enhanced ciprofloxacin efflux.

PMID:
12821500
PMCID:
PMC161847
DOI:
10.1128/aac.47.7.2362-2365.2003
[Indexed for MEDLINE]
Free PMC Article

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