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FEBS Lett. 2002 Feb 13;512(1-3):235-9.

Imaging of the fluorescence spectrum of a single fluorescent molecule by prism-based spectroscopy.

Author information

1
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, 153-8902, Tokyo, Japan.

Abstract

We have devised a novel method to visualize the fluorescence spectrum of a single fluorescent molecule using prism-based spectroscopy. Equipping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin. We also obtained a spectral image of single-pair fluorescence resonance energy transfer between rhodamine red and Cy5 in a double-labeled myosin motor domain. This method could become a useful tool to investigate the dynamic processes of biomolecules at the single-molecule level.

PMID:
11852087
[Indexed for MEDLINE]
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