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Biochem Biophys Res Commun. 2001 May 4;283(2):507-12.

Downregulation of the cdc2/cyclin B protein kinase activity by binding of p53 to p34(cdc2).

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Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, Homburg, 66421, Germany.


We previously found that p53 binds to the catalytic subunit of the p34(cdc2)/cyclin B1-kinase. In the present study we analyzed the functional consequences of this interaction. Binding of wild-type p53 to p34(cdc2)/cyclin B1 results in a significant decrease of its histone H1 kinase activity. Binding of p53 to the kinase is a prerequisite for the inhibition because a mutant p53 which lacks the binding region fails to influence the enzymatic activity. Furthermore, by using C-terminal fragments of p53 it became obvious that also some other structural elements in the N-terminal region are necessary for the inhibitory effect. Our present study provides evidence that p53 might regulate cell-cycle checkpoints not only on the transcriptional level but also by binding to the cell-cycle regulating kinase p34(cdc2).

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