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Proteomics. 2016 Dec;16(23):2962-2966. doi: 10.1002/pmic.201600315. Epub 2016 Nov 9.

Exploring the oviductal fluid proteome by a lectin-based affinity approach.

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Institute of Biotechnology in Animal Production, Interuniversity Department for Agrobiotechnology (IFA Tulln), University of Natural Resources and Applied Life Sciences Vienna, Tulln, Austria.
Institute of Animal Breeding and Genetics, Department for Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, Austria.
Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilian University, Munich, Germany.
Reproduction Centre Wieselburg, University of Veterinary Medicine Vienna, Vienna, Austria.
VetCore Facility for Research, University of Veterinary Medicine Vienna, Vienna, Austria.
Analytical Chemistry, Department of Chemistry-Biomedical Center and Science for Life Laboratory, Uppsala University, Uppsala, Sweden.


The analysis of glycoproteins in body fluids represents a central task in the study of vital processes. Herein, we assessed the combined use of Concanavalin A and Wheat Germ Agglutinin as ligands to fractionate and enrich glycoproteins from oviductal fluid (OF), which is a source of molecules involved in fertilization. First, the selectivity was corroborated by a gel-based approach using glycoprotein staining and enzymatic deglycosylation. Nanoliquid chromatography-tandem mass spectrometry (nLC-ESI-MS/MS) further allowed the reliable identification of 134 nonbound as well as 130 lectin-bound OF proteins. Enrichment analysis revealed that 77% of the annotated proteins in the lectin-bound fraction were known glycoproteins (p-value [FDR] = 1.45E-31). The low variance of the number of peptide spectrum matches for each protein within replicates indicated a consistent reproducibility of the whole workflow (median CV 17.3% for technical replicates and 20.7% for biological replicates). Taken together, this study highlights the applicability of a lectin-based workflow for the comprehensive analysis of OF proteins and gives for the first time an insight into the broad glycoprotein content of OF.


Concanavalin A; Glycoproteins; Oviductal fluid; Technology; Wheat germ agglutinin

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