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Anim Biotechnol. 2001 Nov;12(2):141-4.

DNA microsatellite analysis for parentage control in Austrian pigs.

Author information

1
Institute for Animal Breeding and Genetics, Vienna University of Veterinary Medicine, Austria.

Abstract

We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (PiƩtrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair ("parentage control", e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (PiƩtrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.

PMID:
11808629
DOI:
10.1081/ABIO-100108340
[Indexed for MEDLINE]

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