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Items: 8


Functional Genetic Elements for Controlling Gene Expression in Cupriavidus necator H16.

Alagesan S, Hanko EKR, Malys N, Ehsaan M, Winzer K, Minton NP.

Appl Environ Microbiol. 2018 Sep 17;84(19). pii: e00878-18. doi: 10.1128/AEM.00878-18. Print 2018 Oct 1.


Clostridium difficile Genome Editing Using pyrE Alleles.

Ehsaan M, Kuehne SA, Minton NP.

Methods Mol Biol. 2016;1476:35-52. doi: 10.1007/978-1-4939-6361-4_4.


A roadmap for gene system development in Clostridium.

Minton NP, Ehsaan M, Humphreys CM, Little GT, Baker J, Henstra AM, Liew F, Kelly ML, Sheng L, Schwarz K, Zhang Y.

Anaerobe. 2016 Oct;41:104-112. doi: 10.1016/j.anaerobe.2016.05.011. Epub 2016 May 24. Review.


Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.

Ehsaan M, Kuit W, Zhang Y, Cartman ST, Heap JT, Winzer K, Minton NP.

Biotechnol Biofuels. 2016 Jan 4;9:4. doi: 10.1186/s13068-015-0410-0. eCollection 2016.


Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo.

Heap JT, Theys J, Ehsaan M, Kubiak AM, Dubois L, Paesmans K, Van Mellaert L, Knox R, Kuehne SA, Lambin P, Minton NP.

Oncotarget. 2014 Apr 15;5(7):1761-9.


Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles.

Ng YK, Ehsaan M, Philip S, Collery MM, Janoir C, Collignon A, Cartman ST, Minton NP.

PLoS One. 2013;8(2):e56051. doi: 10.1371/journal.pone.0056051. Epub 2013 Feb 6.


Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton NP.

Nucleic Acids Res. 2012 Apr;40(8):e59. doi: 10.1093/nar/gkr1321. Epub 2012 Jan 18.


The ClosTron: Mutagenesis in Clostridium refined and streamlined.

Heap JT, Kuehne SA, Ehsaan M, Cartman ST, Cooksley CM, Scott JC, Minton NP.

J Microbiol Methods. 2010 Jan;80(1):49-55. doi: 10.1016/j.mimet.2009.10.018. Epub 2009 Nov 3.


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