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Items: 7

1.

DnaQ exonuclease-like domain of Cas2 promotes spacer integration in a type I-E CRISPR-Cas system.

Drabavicius G, Sinkunas T, Silanskas A, Gasiunas G, Venclovas Č, Siksnys V.

EMBO Rep. 2018 Jun 11. pii: e45543. doi: 10.15252/embr.201745543. [Epub ahead of print]

PMID:
29891635
2.

Cas3 nuclease-helicase activity assays.

Sinkunas T, Gasiunas G, Siksnys V.

Methods Mol Biol. 2015;1311:277-91. doi: 10.1007/978-1-4939-2687-9_18.

PMID:
25981480
3.

Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection.

Rutkauskas M, Sinkunas T, Songailiene I, Tikhomirova MS, Siksnys V, Seidel R.

Cell Rep. 2015 Mar 3. pii: S2211-1247(15)00135-7. doi: 10.1016/j.celrep.2015.01.067. [Epub ahead of print]

4.

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

Szczelkun MD, Tikhomirova MS, Sinkunas T, Gasiunas G, Karvelis T, Pschera P, Siksnys V, Seidel R.

Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9798-803. doi: 10.1073/pnas.1402597111. Epub 2014 May 27.

5.

Molecular mechanisms of CRISPR-mediated microbial immunity.

Gasiunas G, Sinkunas T, Siksnys V.

Cell Mol Life Sci. 2014 Feb;71(3):449-65. Review.

6.

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus.

Sinkunas T, Gasiunas G, Waghmare SP, Dickman MJ, Barrangou R, Horvath P, Siksnys V.

EMBO J. 2013 Feb 6;32(3):385-94. doi: 10.1038/emboj.2012.352. Epub 2013 Jan 18.

7.

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system.

Sinkunas T, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V.

EMBO J. 2011 Apr 6;30(7):1335-42. doi: 10.1038/emboj.2011.41. Epub 2011 Feb 22.

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