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Int J Exp Pathol. 2004 Apr;85(2):65-74.

Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines.

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Department of Histopathology, Hammersmith Hospital, Imperial College of Science, Technology and Medicine, London, UK.

Erratum in

  • Int J Exp Pathol. 2004 Jun;85(3):175. Poulsom, S Richard [corrected to Poulsom, Richard]; Tomlinson, Ian T [corrected to Tomlinson, Ian T M].


The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. Beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations.

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