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Finally, Archaea Get Their CRISPR-Cas Toolbox.

Gophna U, Allers T, Marchfelder A.

Trends Microbiol. 2017 Jun;25(6):430-432. doi: 10.1016/j.tim.2017.03.009. Epub 2017 Apr 6.


The crystal structure of Trz1, the long form RNase Z from yeast.

Ma M, Li de la Sierra-Gallay I, Lazar N, Pellegrini O, Durand D, Marchfelder A, Condon C, van Tilbeurgh H.

Nucleic Acids Res. 2017 Jun 2;45(10):6209-6216. doi: 10.1093/nar/gkx216.


High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.

Stachler AE, Turgeman-Grott I, Shtifman-Segal E, Allers T, Marchfelder A, Gophna U.

Nucleic Acids Res. 2017 May 19;45(9):5208-5216. doi: 10.1093/nar/gkx150.


Genome-wide identification of transcriptional start sites in the haloarchaeon Haloferax volcanii based on differential RNA-Seq (dRNA-Seq).

Babski J, Haas KA, Näther-Schindler D, Pfeiffer F, Förstner KU, Hammelmann M, Hilker R, Becker A, Sharma CM, Marchfelder A, Soppa J.

BMC Genomics. 2016 Aug 12;17(1):629. doi: 10.1186/s12864-016-2920-y.


Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System.

Stachler AE, Marchfelder A.

J Biol Chem. 2016 Jul 15;291(29):15226-42. doi: 10.1074/jbc.M116.724062. Epub 2016 May 16.


The role of Cas8 in type I CRISPR interference.

Cass SD, Haas KA, Stoll B, Alkhnbashi OS, Sharma K, Urlaub H, Backofen R, Marchfelder A, Bolt EL.

Biosci Rep. 2015 May 5;35(3). pii: e00197. doi: 10.1042/BSR20150043.


Analysis of protein-RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry.

Sharma K, Hrle A, Kramer K, Sachsenberg T, Staals RH, Randau L, Marchfelder A, van der Oost J, Kohlbacher O, Conti E, Urlaub H.

Methods. 2015 Nov 1;89:138-48. doi: 10.1016/j.ymeth.2015.06.005. Epub 2015 Jun 10.


Deletion of the Sm1 encoding motif in the lsm gene results in distinct changes in the transcriptome and enhanced swarming activity of Haloferax cells.

Maier LK, Benz J, Fischer S, Alstetter M, Jaschinski K, Hilker R, Becker A, Allers T, Soppa J, Marchfelder A.

Biochimie. 2015 Oct;117:129-37. doi: 10.1016/j.biochi.2015.02.023. Epub 2015 Mar 6.


The Adaptive Immune System of Haloferax volcanii.

Maier LK, Dyall-Smith M, Marchfelder A.

Life (Basel). 2015 Feb 16;5(1):521-37. doi: 10.3390/life5010521. Review.


An active immune defense with a minimal CRISPR (clustered regularly interspaced short palindromic repeats) RNA and without the Cas6 protein.

Maier LK, Stachler AE, Saunders SJ, Backofen R, Marchfelder A.

J Biol Chem. 2015 Feb 13;290(7):4192-201. doi: 10.1074/jbc.M114.617506. Epub 2014 Dec 15.


Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.

Hrle A, Maier LK, Sharma K, Ebert J, Basquin C, Urlaub H, Marchfelder A, Conti E.

RNA Biol. 2014;11(8):1072-82. doi: 10.4161/rna.29893.


Small regulatory RNAs in Archaea.

Babski J, Maier LK, Heyer R, Jaschinski K, Prasse D, Jäger D, Randau L, Schmitz RA, Marchfelder A, Soppa J.

RNA Biol. 2014;11(5):484-93. Epub 2014 Mar 31. Review.


Generation and phenotyping of a collection of sRNA gene deletion mutants of the haloarchaeon Haloferax volcanii.

Jaschinski K, Babski J, Lehr M, Burmester A, Benz J, Heyer R, Dörr M, Marchfelder A, Soppa J.

PLoS One. 2014 Mar 17;9(3):e90763. doi: 10.1371/journal.pone.0090763. eCollection 2014.


A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii.

Brendel J, Stoll B, Lange SJ, Sharma K, Lenz C, Stachler AE, Maier LK, Richter H, Nickel L, Schmitz RA, Randau L, Allers T, Urlaub H, Backofen R, Marchfelder A.

J Biol Chem. 2014 Mar 7;289(10):7164-77. doi: 10.1074/jbc.M113.508184. Epub 2014 Jan 23.


Requirements for a successful defence reaction by the CRISPR-Cas subtype I-B system.

Stoll B, Maier LK, Lange SJ, Brendel J, Fischer S, Backofen R, Marchfelder A.

Biochem Soc Trans. 2013 Dec;41(6):1444-8. doi: 10.1042/BST20130098. Review.


tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6.

Skowronek E, Grzechnik P, Späth B, Marchfelder A, Kufel J.

RNA. 2014 Jan;20(1):115-30. doi: 10.1261/rna.041467.113. Epub 2013 Nov 18.


RNA-Pareto: interactive analysis of Pareto-optimal RNA sequence-structure alignments.

Schnattinger T, Schöning U, Marchfelder A, Kestler HA.

Bioinformatics. 2013 Dec 1;29(23):3102-4. doi: 10.1093/bioinformatics/btt536. Epub 2013 Sep 16.


Special focus CRISPR-Cas.

Marchfelder A.

RNA Biol. 2013 May;10(5):655-8. doi: 10.4161/rna.24687. No abstract available.


Essential requirements for the detection and degradation of invaders by the Haloferax volcanii CRISPR/Cas system I-B.

Maier LK, Lange SJ, Stoll B, Haas KA, Fischer S, Fischer E, Duchardt-Ferner E, Wöhnert J, Backofen R, Marchfelder A.

RNA Biol. 2013 May;10(5):865-74. doi: 10.4161/rna.24282. Epub 2013 Apr 17.


The immune system of halophilic archaea.

Maier LK, Fischer S, Stoll B, Brendel J, Pfeiffer F, Dyall-Smith M, Marchfelder A.

Mob Genet Elements. 2012 Sep 1;2(5):228-232.


The ring of confidence: a haloarchaeal CRISPR/Cas system.

Maier LK, Stoll B, Brendel J, Fischer S, Pfeiffer F, Dyall-Smith M, Marchfelder A.

Biochem Soc Trans. 2013 Feb 1;41(1):374-8. doi: 10.1042/BST20120263.


An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA.

Fischer S, Maier LK, Stoll B, Brendel J, Fischer E, Pfeiffer F, Dyall-Smith M, Marchfelder A.

J Biol Chem. 2012 Sep 28;287(40):33351-63. Epub 2012 Jul 5.


High throughput sequencing reveals a plethora of small RNAs including tRNA derived fragments in Haloferax volcanii.

Heyer R, Dörr M, Jellen-Ritter A, Späth B, Babski J, Jaschinski K, Soppa J, Marchfelder A.

RNA Biol. 2012 Jul;9(7):1011-8. doi: 10.4161/rna.20826. Epub 2012 Jul 1.


Small RNAs for defence and regulation in archaea.

Marchfelder A, Fischer S, Brendel J, Stoll B, Maier LK, Jäger D, Prasse D, Plagens A, Schmitz RA, Randau L.

Extremophiles. 2012 Sep;16(5):685-96. doi: 10.1007/s00792-012-0469-5. Epub 2012 Jul 5. Review.


Complex I-complex II ratio strongly differs in various organs of Arabidopsis thaliana.

Peters K, Niessen M, Peterhänsel C, Späth B, Hölzle A, Binder S, Marchfelder A, Braun HP.

Plant Mol Biol. 2012 Jun;79(3):273-84. doi: 10.1007/s11103-012-9911-4. Epub 2012 Apr 17.


Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii.

Fischer S, John von Freyend S, Sabag-Daigle A, Daniels CJ, Allers T, Marchfelder A.

Extremophiles. 2012 Mar;16(2):333-43. doi: 10.1007/s00792-012-0433-4. Epub 2012 Feb 18.


tRNA-like elements in Haloferax volcanii.

Hölzle A, Stoll B, Schnattinger T, Schöning U, Tjaden B, Marchfelder A.

Biochimie. 2012 Apr;94(4):940-6. doi: 10.1016/j.biochi.2011.12.002. Epub 2011 Dec 8.


Regulatory RNAs and beyond.

Hammann C, Hartmann RK, Helm M, Klostermeier D, Marchfelder A, Suess B, Vörtler S.

EMBO Rep. 2011 Jul 15;12(8):751-3. doi: 10.1038/embor.2011.150.


Bioinformatic prediction and experimental verification of sRNAs in the haloarchaeon Haloferax volcanii.

Babski J, Tjaden B, Voss B, Jellen-Ritter A, Marchfelder A, Hess WR, Soppa J.

RNA Biol. 2011 Sep-Oct;8(5):806-16. doi: 10.4161/rna.8.5.16039. Epub 2011 Jun 29.


Regulatory RNAs in Haloferax volcanii.

Fischer S, Benz J, Späth B, Jellen-Ritter A, Heyer R, Dörr M, Maier LK, Menzel-Hobeck C, Lehr M, Jantzer K, Babski J, Soppa J, Marchfelder A.

Biochem Soc Trans. 2011 Jan;39(1):159-62. doi: 10.1042/BST0390159.


The archaeal Lsm protein binds to small RNAs.

Fischer S, Benz J, Späth B, Maier LK, Straub J, Granzow M, Raabe M, Urlaub H, Hoffmann J, Brutschy B, Allers T, Soppa J, Marchfelder A.

J Biol Chem. 2010 Nov 5;285(45):34429-38. doi: 10.1074/jbc.M110.118950. Epub 2010 Sep 7.


Processing of a dicistronic tRNA-snoRNA precursor: combined analysis in vitro and in vivo reveals alternate pathways and coupling to assembly of snoRNP.

Barbezier N, Canino G, Rodor J, Jobet E, Saez-Vasquez J, Marchfelder A, Echeverría M.

Plant Physiol. 2009 Jul;150(3):1598-610. doi: 10.1104/pp.109.137968. Epub 2009 May 6.


Arabidopsis encodes four tRNase Z enzymes.

Canino G, Bocian E, Barbezier N, Echeverría M, Forner J, Binder S, Marchfelder A.

Plant Physiol. 2009 Jul;150(3):1494-502. doi: 10.1104/pp.109.137950. Epub 2009 May 1.


Small RNAs in haloarchaea: identification, differential expression and biological function.

Straub J, Brenneis M, Jellen-Ritter A, Heyer R, Soppa J, Marchfelder A.

RNA Biol. 2009 Jul-Aug;6(3):281-92. Epub 2009 Jul 6.


The making of tRNAs and more - RNase P and tRNase Z.

Hartmann RK, Gössringer M, Späth B, Fischer S, Marchfelder A.

Prog Mol Biol Transl Sci. 2009;85:319-68. doi: 10.1016/S0079-6603(08)00808-8. Review.


Small RNAs of the halophilic archaeon Haloferax volcanii.

Soppa J, Straub J, Brenneis M, Jellen-Ritter A, Heyer R, Fischer S, Granzow M, Voss B, Hess WR, Tjaden B, Marchfelder A.

Biochem Soc Trans. 2009 Feb;37(Pt 1):133-6. doi: 10.1042/BST0370133.


Two archaeal tRNase Z enzymes: similar but different.

Späth B, Schubert S, Lieberoth A, Settele F, Schütz S, Fischer S, Marchfelder A.

Arch Microbiol. 2008 Sep;190(3):301-8. doi: 10.1007/s00203-008-0368-4. Epub 2008 Apr 25.


Maturation of the 5S rRNA 5' end is catalyzed in vitro by the endonuclease tRNase Z in the archaeon H. volcanii.

Hölzle A, Fischer S, Heyer R, Schütz S, Zacharias M, Walther P, Allers T, Marchfelder A.

RNA. 2008 May;14(5):928-37. doi: 10.1261/rna.933208. Epub 2008 Mar 27.


Metal requirements and phosphodiesterase activity of tRNase Z enzymes.

Späth B, Settele F, Schilling O, D'Angelo I, Vogel A, Feldmann I, Meyer-Klaucke W, Marchfelder A.

Biochemistry. 2007 Dec 25;46(51):14742-50. Epub 2007 Dec 4.


tRNase Z: the end is not in sight.

Späth B, Canino G, Marchfelder A.

Cell Mol Life Sci. 2007 Sep;64(18):2404-12. Review.


25 years of catalytic RNA: looking younger than ever!

Hammann C, Hartmann RK, Marchfelder A.

Biol Chem. 2007 Jul;388(7):659-60. No abstract available.


The tRNase Z family of proteins: physiological functions, substrate specificity and structural properties.

Vogel A, Schilling O, Späth B, Marchfelder A.

Biol Chem. 2005 Dec;386(12):1253-64. Review.


Highlight: RNA biochemistry.

Marchfelder A, Hartmann RK.

Biol Chem. 2005 Dec;386(12):1203. No abstract available.


Analysis of the functional modules of the tRNA 3' endonuclease (tRNase Z).

Späth B, Kirchner S, Vogel A, Schubert S, Meinlschmidt P, Aymanns S, Nezzar J, Marchfelder A.

J Biol Chem. 2005 Oct 21;280(42):35440-7. Epub 2005 Aug 22.


Exosite modules guide substrate recognition in the ZiPD/ElaC protein family.

Schilling O, Späth B, Kostelecky B, Marchfelder A, Meyer-Klaucke W, Vogel A.

J Biol Chem. 2005 May 6;280(18):17857-62. Epub 2005 Feb 7.


Zinc- and iron-dependent cytosolic metallo-beta-lactamase domain proteins exhibit similar zinc-binding affinities, independent of an atypical glutamate at the metal-binding site.

Schilling O, Vogel A, Kostelecky B, Natal da Luz H, Spemann D, Späth B, Marchfelder A, Tröger W, Meyer-Klaucke W.

Biochem J. 2005 Jan 1;385(Pt 1):145-53.


Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo.

Dubrovsky EB, Dubrovskaya VA, Levinger L, Schiffer S, Marchfelder A.

Nucleic Acids Res. 2004 Jan 9;32(1):255-62. Print 2004.


Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis.

Pellegrini O, Nezzar J, Marchfelder A, Putzer H, Condon C.

EMBO J. 2003 Sep 1;22(17):4534-43.


Plant dicistronic tRNA-snoRNA genes: a new mode of expression of the small nucleolar RNAs processed by RNase Z.

Kruszka K, Barneche F, Guyot R, Ailhas J, Meneau I, Schiffer S, Marchfelder A, Echeverría M.

EMBO J. 2003 Feb 3;22(3):621-32.

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