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EMBO J. 2017 Mar 15;36(6):707-717. doi: 10.15252/embj.201796603. Epub 2017 Feb 22.

Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize.

Author information

1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon ENS de Lyon UCB Lyon 1 CNRS, INRA, Lyon, France.
2
Limagrain Europe SAS, Research Centre, Chappes, France.
3
Department of Genetics, Faculty of Agriculture, Sohag University, Sohag, Egypt.
4
INRA, US1258 Centre National des Ressources Génomiques Végétales, Auzeville, France.
5
INRA, UMR1095 Génétique, Diversité, Ecophysiologie des Céréales, Clermont-Ferrand, France.
6
Laboratoire Reproduction et Développement des Plantes, Univ Lyon ENS de Lyon UCB Lyon 1 CNRS, INRA, Lyon, France thomas.widiez@ens-lyon.fr.

Abstract

Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type NLD abolishes the haploid induction capacity. Activity of the NLD promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In Arabidopsis roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.

KEYWORDS:

Zea mays ; embryo; fertilization; gynogenesis; haploid; phospholipase

Comment in

PMID:
28228439
PMCID:
PMC5350562
DOI:
10.15252/embj.201796603
[Indexed for MEDLINE]
Free PMC Article

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