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RNA. 2012 Oct;18(10):1796-804. doi: 10.1261/rna.031278.111. Epub 2012 Aug 21.

Single-stranded microRNA mimics.

Author information

1
Sirna Therapeutics, a wholly owned subsidiary of Merck & Co., San Francisco, California 94158, USA.

Abstract

miRNAs are ∼22-nt RNAs that bind to the Argonaute family of proteins and have important regulatory roles in plants and animals. Here, we show that miRNAs exhibit targeting activity in cells when delivered as single strands that are 5'-phosphorylated and that contain 2'-fluoro ribose modifications. Length preferences, chemical modification sensitivity, and genome-wide seed-based targeting all suggest that this activity is Ago-based. Activity could be enhanced by annealing of segmented passenger strands containing non-nucleic acid spacers. Furthermore, screening of randomly generated sequences identified pyrimidine rich 3' cassette sequences that increased single strand activity. These results provide an initial step in the development of single-stranded miRNA mimics for therapeutic use.

PMID:
22912485
PMCID:
PMC3446704
DOI:
10.1261/rna.031278.111
[Indexed for MEDLINE]
Free PMC Article

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