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Items: 1 to 50 of 90

1.

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

Gale D, Lawson ARJ, Howarth K, Madi M, Durham B, Smalley S, Calaway J, Blais S, Jones G, Clark J, Dimitrov P, Pugh M, Woodhouse S, Epstein M, Fernandez-Gonzalez A, Whale AS, Huggett JF, Foy CA, Jones GM, Raveh-Amit H, Schmitt K, Devonshire A, Green E, Forshew T, Plagnol V, Rosenfeld N.

PLoS One. 2018 Mar 16;13(3):e0194630. doi: 10.1371/journal.pone.0194630. eCollection 2018.

2.

qPCR primer design revisited.

Bustin S, Huggett J.

Biomol Detect Quantif. 2017 Nov 22;14:19-28. doi: 10.1016/j.bdq.2017.11.001. eCollection 2017 Dec. Review.

3.

G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon.

Dombrowski JG, Souza RM, Curry J, Hinton L, Silva NRM, Grignard L, Gonçalves LA, Gomes AR, Epiphanio S, Drakeley C, Huggett J, Clark TG, Campino S, Marinho CRF.

Malar J. 2017 Jun 15;16(1):253. doi: 10.1186/s12936-017-1889-6.

4.

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

Busby E, Whale AS, Ferns RB, Grant PR, Morley G, Campbell J, Foy CA, Nastouli E, Huggett JF, Garson JA.

Sci Rep. 2017 Apr 26;7(1):1209. doi: 10.1038/s41598-017-01221-5.

5.

Reproducibility of biomedical research - The importance of editorial vigilance.

Bustin SA, Huggett JF.

Biomol Detect Quantif. 2017 Feb 21;11:1-3. doi: 10.1016/j.bdq.2017.01.002. eCollection 2017 Mar.

6.

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

Yoo HB, Park SR, Dong L, Wang J, Sui Z, Pavšič J, Milavec M, Akgoz M, Mozioğlu E, Corbisier P, Janka M, Cosme B, de V Cavalcante JJ, Flatshart RB, Burke D, Forbes-Smith M, McLaughlin J, Emslie K, Whale AS, Huggett JF, Parkes H, Kline MC, Harenza JL, Vallone PM.

Anal Chem. 2016 Dec 20;88(24):12169-12176. doi: 10.1021/acs.analchem.6b03076. Epub 2016 Nov 30.

PMID:
28193036
7.

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

Pavšič J, Devonshire A, Blejec A, Foy CA, Van Heuverswyn F, Jones GM, Schimmel H, Žel J, Huggett JF, Redshaw N, Karczmarczyk M, Mozioğlu E, Akyürek S, Akgöz M, Milavec M.

Anal Bioanal Chem. 2017 Apr;409(10):2601-2614. doi: 10.1007/s00216-017-0206-0. Epub 2017 Jan 26.

8.

The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs.

Phelan J, O'Sullivan DM, Machado D, Ramos J, Whale AS, O'Grady J, Dheda K, Campino S, McNerney R, Viveiros M, Huggett JF, Clark TG.

Genome Med. 2016 Dec 22;8(1):132. doi: 10.1186/s13073-016-0385-x.

9.

Fundamentals of multiplexing with digital PCR.

Whale AS, Huggett JF, Tzonev S.

Biomol Detect Quantif. 2016 May 27;10:15-23. eCollection 2016 Dec. Review.

10.

Digital PCR, a technique for the future

Taly V, Huggett J.

Biomol Detect Quantif. 2016 Nov 25;10:1. eCollection 2016 Dec. No abstract available.

11.

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia.

Alikian M, Whale AS, Akiki S, Piechocki K, Torrado C, Myint T, Cowen S, Griffiths M, Reid AG, Apperley J, White H, Huggett JF, Foroni L.

Clin Chem. 2017 Feb;63(2):525-531. doi: 10.1373/clinchem.2016.262824. Epub 2016 Dec 15.

12.

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant.

Whale AS, Devonshire AS, Karlin-Neumann G, Regan J, Javier L, Cowen S, Fernandez-Gonzalez A, Jones GM, Redshaw N, Beck J, Berger AW, Combaret V, Dahl Kjersgaard N, Davis L, Fina F, Forshew T, Fredslund Andersen R, Galbiati S, González Hernández Á, Haynes CA, Janku F, Lacave R, Lee J, Mistry V, Pender A, Pradines A, Proudhon C, Saal LH, Stieglitz E, Ulrich B, Foy CA, Parkes H, Tzonev S, Huggett JF.

Anal Chem. 2017 Feb 7;89(3):1724-1733. doi: 10.1021/acs.analchem.6b03980. Epub 2017 Jan 18.

PMID:
27935690
13.

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis.

Devonshire AS, O'Sullivan DM, Honeyborne I, Jones G, Karczmarczyk M, Pavšič J, Gutteridge A, Milavec M, Mendoza P, Schimmel H, Van Heuverswyn F, Gorton R, Cirillo DM, Borroni E, Harris K, Barnard M, Heydenrych A, Ndusilo N, Wallis CL, Pillay K, Barry T, Reddington K, Richter E, Mozioğlu E, Akyürek S, Yalçınkaya B, Akgoz M, Žel J, Foy CA, McHugh TD, Huggett JF.

BMC Infect Dis. 2016 Aug 3;16:366. doi: 10.1186/s12879-016-1696-7.

14.

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

Devonshire AS, Sanders R, Whale AS, Nixon GJ, Cowen S, Ellison SL, Parkes H, Pine PS, Salit M, McDaniel J, Munro S, Lund S, Matsukura S, Sekiguchi Y, Kawaharasaki M, Granjeiro JM, Falagan-Lotsch P, Saraiva AM, Couto P, Yang I, Kwon H, Park SR, Demšar T, Žel J, Blejec A, Milavec M, Dong L, Zhang L, Sui Z, Wang J, Viroonudomphol D, Prawettongsopon C, Partis L, Baoutina A, Emslie K, Takatsu A, Akyurek S, Akgoz M, Vonsky M, Konopelko LA, Cundapi EM, Urquiza MP, Huggett JF, Foy CA.

Biomol Detect Quantif. 2016 Jun 6;8:15-28. doi: 10.1016/j.bdq.2016.05.003. eCollection 2016 Jun.

15.

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL.

Camacho F, Sarmiento ME, Reyes F, Kim L, Huggett J, Lepore M, Otero O, Gilleron M, Puzo G, Norazmi MN, Rook G, Mori L, De Libero G, Acosta A.

Int J Mycobacteriol. 2016 Jun;5(2):120-7. doi: 10.1016/j.ijmyco.2015.12.002. Epub 2016 Feb 17.

16.

qPCR, dPCR, NGS - A journey.

Huggett JF, O'Grady J, Bustin S.

Biomol Detect Quantif. 2015 Jan 15;3:A1-5. doi: 10.1016/j.bdq.2015.01.001. eCollection 2015 Mar. No abstract available.

17.

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons.

Olson ND, Lund SP, Zook JM, Rojas-Cornejo F, Beck B, Foy C, Huggett J, Whale AS, Sui Z, Baoutina A, Dobeson M, Partis L, Morrow JB.

Biomol Detect Quantif. 2015 Mar 5;3:17-24. doi: 10.1016/j.bdq.2015.01.004. eCollection 2015 Mar.

18.

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.

Alikian M, Ellery P, Forbes M, Gerrard G, Kasperaviciute D, Sosinsky A, Mueller M, Whale AS, Milojkovic D, Apperley J, Huggett JF, Foroni L, Reid AG.

J Mol Diagn. 2016 Mar;18(2):176-89. doi: 10.1016/j.jmoldx.2015.09.005. Epub 2016 Feb 5.

19.

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Whale AS, Bushell CA, Grant PR, Cowen S, Gutierrez-Aguirre I, O'Sullivan DM, Žel J, Milavec M, Foy CA, Nastouli E, Garson JA, Huggett JF.

J Clin Microbiol. 2016 Feb;54(2):392-400. doi: 10.1128/JCM.02611-15. Epub 2015 Dec 9.

20.

[The digital polymerase chain reaction as a new technology and its potential input in molecular diagnostic].

Huggett JF, Whale AS.

Klin Lab Diagn. 2014 Nov;59(11):6-8. Russian. No abstract available.

PMID:
25850236
21.

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

Devonshire AS, Honeyborne I, Gutteridge A, Whale AS, Nixon G, Wilson P, Jones G, McHugh TD, Foy CA, Huggett JF.

Anal Chem. 2015 Apr 7;87(7):3706-13. doi: 10.1021/ac5041617. Epub 2015 Mar 10.

PMID:
25646934
22.

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis.

Redshaw N, Huggett JF, Taylor MS, Foy CA, Devonshire AS.

BMC Genomics. 2014 Dec 23;15:1174. doi: 10.1186/1471-2164-15-1174.

23.

Making standards for quantitative real-time pneumococcal PCR.

Morpeth SC, Huggett JF, Murdoch DR, Scott JA.

Biomol Detect Quantif. 2014 Dec 4;2:1-3. eCollection 2014 Dec.

24.

Assessing the accuracy of quantitative molecular microbial profiling.

O'Sullivan DM, Laver T, Temisak S, Redshaw N, Harris KA, Foy CA, Studholme DJ, Huggett JF.

Int J Mol Sci. 2014 Nov 21;15(11):21476-91. doi: 10.3390/ijms151121476.

25.

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

Nixon GJ, Svenstrup HF, Donald CE, Carder C, Stephenson JM, Morris-Jones S, Huggett JF, Foy CA.

Biomol Detect Quantif. 2014 Nov 22;2:4-10. eCollection 2014 Dec.

26.

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

Pavšič J, Devonshire AS, Parkes H, Schimmel H, Foy CA, Karczmarczyk M, Gutiérrez-Aguirre I, Honeyborne I, Huggett JF, McHugh TD, Milavec M, Zeichhardt H, Žel J.

J Clin Microbiol. 2015 Jul;53(7):2008-14. doi: 10.1128/JCM.02136-14. Epub 2014 Nov 12. Review.

27.

Considerations for digital PCR as an accurate molecular diagnostic tool.

Huggett JF, Cowen S, Foy CA.

Clin Chem. 2015 Jan;61(1):79-88. doi: 10.1373/clinchem.2014.221366. Epub 2014 Oct 22. Review.

28.

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

Marks DJ, Hyams C, Koo CY, Pavlou M, Robbins J, Koo CS, Rodger G, Huggett JF, Yap J, Macrae MB, Swanton RH, Zumla AI, Miller RF.

QJM. 2015 Mar;108(3):219-29. doi: 10.1093/qjmed/hcu188. Epub 2014 Sep 15.

PMID:
25223570
29.

How to make Mathematics Biology's next and better microscope.

Huggett J, O'Grady J, Bustin S.

Biomol Detect Quantif. 2014 Sep 10;1(1):A1-A3. eCollection 2014 Sep. No abstract available.

30.

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.

Sanders R, Mason DJ, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6471-83. doi: 10.1007/s00216-014-7857-x. Epub 2014 May 25. Review.

31.

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification.

Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6499-512. doi: 10.1007/s00216-014-7835-3. Epub 2014 May 24.

32.

Minimum information necessary for quantitative real-time PCR experiments.

Johnson G, Nour AA, Nolan T, Huggett J, Bustin S.

Methods Mol Biol. 2014;1160:5-17. doi: 10.1007/978-1-4939-0733-5_2.

PMID:
24740217
33.
34.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
35.

Digital PCR as a novel technology and its potential implications for molecular diagnostics.

Huggett JF, Whale A.

Clin Chem. 2013 Dec;59(12):1691-3. doi: 10.1373/clinchem.2013.214742. Epub 2013 Oct 7. No abstract available.

36.

Evaluation of digital PCR for absolute RNA quantification.

Sanders R, Mason DJ, Foy CA, Huggett JF.

PLoS One. 2013 Sep 20;8(9):e75296. doi: 10.1371/journal.pone.0075296. eCollection 2013.

37.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

38.

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells.

Camacho F, Huggett J, Kim L, Infante JF, Lepore M, Perez V, Sarmiento ME, Rook G, Acosta A.

BMC Immunol. 2013;14 Suppl 1:S2. doi: 10.1186/1471-2172-14-S1-S2. Epub 2013 Feb 25.

39.

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability.

Redshaw N, Wilkes T, Whale A, Cowen S, Huggett J, Foy CA.

Biotechniques. 2013 Mar;54(3):155-64. doi: 10.2144/000114002.

40.

Methods for applying accurate digital PCR analysis on low copy DNA samples.

Whale AS, Cowen S, Foy CA, Huggett JF.

PLoS One. 2013;8(3):e58177. doi: 10.1371/journal.pone.0058177. Epub 2013 Mar 5.

41.

Progress in metagenomics requires a balanced appraisal of the available technologies.

Huggett JF, Studholme DJ, Laver T, Foy CA.

Eur J Clin Microbiol Infect Dis. 2013 Aug;32(8):1097-8. doi: 10.1007/s10096-013-1842-8. Epub 2013 Feb 23. No abstract available.

PMID:
23435754
42.

A phantom-based evaluation of three commercially available patient organ shields for computed tomography X-ray examinations in diagnostic radiology.

Huggett J, Mukonoweshuro W, Loader R.

Radiat Prot Dosimetry. 2013 Jul;155(2):161-8. doi: 10.1093/rpd/ncs327. Epub 2012 Dec 5.

PMID:
23222552
43.

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis.

Devonshire AS, Sanders R, Wilkes TM, Taylor MS, Foy CA, Huggett JF.

Methods. 2013 Jan;59(1):89-100. doi: 10.1016/j.ymeth.2012.07.021. Epub 2012 Jul 24. Review.

PMID:
22841564
44.

Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis.

Wahlberg K, Huggett J, Sanders R, Whale AS, Bushell C, Elaswarapu R, Scott DJ, Foy CA.

Biopreserv Biobank. 2012 Jun;10(3):266-75. doi: 10.1089/bio.2012.0004.

PMID:
24835065
45.

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.

Whale AS, Huggett JF, Cowen S, Speirs V, Shaw J, Ellison S, Foy CA, Scott DJ.

Nucleic Acids Res. 2012 Jun;40(11):e82. doi: 10.1093/nar/gks203. Epub 2012 Feb 28.

46.

Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study.

Rachow A, Zumla A, Heinrich N, Rojas-Ponce G, Mtafya B, Reither K, Ntinginya EN, O'Grady J, Huggett J, Dheda K, Boehme C, Perkins M, Saathoff E, Hoelscher M.

PLoS One. 2011;6(6):e20458. doi: 10.1371/journal.pone.0020458. Epub 2011 Jun 29.

47.

Evaluation of digital PCR for absolute DNA quantification.

Sanders R, Huggett JF, Bushell CA, Cowen S, Scott DJ, Foy CA.

Anal Chem. 2011 Sep 1;83(17):6474-84. doi: 10.1021/ac103230c. Epub 2011 Aug 1.

PMID:
21446772
48.

Primer sequence disclosure: a clarification of the MIQE guidelines.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT.

Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18. No abstract available.

49.

Accommodating dynamic oceanographic processes and pelagic biodiversity in marine conservation planning.

Grantham HS, Game ET, Lombard AT, Hobday AJ, Richardson AJ, Beckley LE, Pressey RL, Huggett JA, Coetzee JC, van der Lingen CD, Petersen SL, Merkle D, Possingham HP.

PLoS One. 2011 Feb 2;6(2):e16552. doi: 10.1371/journal.pone.0016552.

50.

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments.

Bustin SA, Beaulieu JF, Huggett J, Jaggi R, Kibenge FS, Olsvik PA, Penning LC, Toegel S.

BMC Mol Biol. 2010 Sep 21;11:74. doi: 10.1186/1471-2199-11-74.

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