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Items: 1 to 50 of 226

1.

The use of whole-genome sequencing in cluster investigation of an MDR-TB outbreak.

Lalor MK, Casali N, Walker TM, Anderson LF, Davidson JA, Ratna N, Mullarkey C, Gent M, Foster K, Brown T, Magee J, Barrett A, Crook DW, Drobniewski F, Thomas HL, Abubakar I.

Eur Respir J. 2018 May 10. pii: 1702313. doi: 10.1183/13993003.02313-2017. [Epub ahead of print] No abstract available.

PMID:
29748309
2.

Patient and strain characteristics associated with Clostridium difficile transmission and adverse outcomes.

Martin JSH, Eyre DW, Fawley WN, Griffiths D, Davies K, Mawer DPC, Peto TEA, Crook DW, Walker AS, Wilcox MH.

Clin Infect Dis. 2018 Apr 12. doi: 10.1093/cid/ciy302. [Epub ahead of print]

PMID:
29659753
3.

Two Distinct Patterns of Clostridium Difficile Diversity Across Europe Indicates Contrasting Routes of Spread.

Eyre DW, Davies KA, Davis G, Fawley WN, Dingle KE, De Maio N, Karas A, Crook DW, Peto TEA, Walker AS, Wilcox MH; EUCLID study group.

Clin Infect Dis. 2018 Apr 6. doi: 10.1093/cid/ciy252. [Epub ahead of print]

PMID:
29659747
4.

DNA extraction from primary liquid blood cultures for bloodstream infection diagnosis using whole genome sequencing.

Anson LW, Chau K, Sanderson N, Hoosdally S, Bradley P, Iqbal Z, Phan H, Foster D, Oakley S, Morgan M, Peto TEA, Modernizing Medical Microbiology Informatics Group Mmmig, Crook DW, Pankhurst LJ.

J Med Microbiol. 2018 Mar;67(3):347-357. doi: 10.1099/jmm.0.000664. Epub 2018 Jan 10.

5.

Intensive Care Unit Wastewater Interventions to Prevent Transmission of Multi-species Klebsiella pneumoniae Carbapenemase (KPC) Producing Organisms.

Mathers AJ, Vegesana K, German Mesner I, Barry KE, Pannone A, Baumann J, Crook DW, Stoesser N, Kotay S, Carroll J, Sifri CD.

Clin Infect Dis. 2018 Feb 2. doi: 10.1093/cid/ciy052. [Epub ahead of print]

PMID:
29409044
6.

Robust Prediction of Resistance to Trimethoprim in Staphylococcus aureus.

Fowler PW, Cole K, Gordon NC, Kearns AM, Llewelyn MJ, Peto TEA, Crook DW, Walker AS.

Cell Chem Biol. 2018 Mar 15;25(3):339-349.e4. doi: 10.1016/j.chembiol.2017.12.009. Epub 2018 Jan 4.

PMID:
29307840
7.

Severe infections emerge from commensal bacteria by adaptive evolution.

Young BC, Wu CH, Gordon NC, Cole K, Price JR, Liu E, Sheppard AE, Perera S, Charlesworth J, Golubchik T, Iqbal Z, Bowden R, Massey RC, Paul J, Crook DW, Peto TE, Walker AS, Llewelyn MJ, Wyllie DH, Wilson DJ.

Elife. 2017 Dec 19;6. pii: e30637. doi: 10.7554/eLife.30637.

8.

Machine learning for classifying tuberculosis drug-resistance from DNA sequencing data.

Yang Y, Niehaus KE, Walker TM, Iqbal Z, Walker AS, Wilson DJ, Peto TEA, Crook DW, Smith EG, Zhu T, Clifton DA.

Bioinformatics. 2018 May 15;34(10):1666-1671. doi: 10.1093/bioinformatics/btx801.

9.

Illumina short-read and MinION long-read WGS to characterize the molecular epidemiology of an NDM-1 Serratia marcescens outbreak in Romania.

Phan HTT, Stoesser N, Maciuca IE, Toma F, Szekely E, Flonta M, Hubbard ATM, Pankhurst L, Do T, Peto TEA, Walker AS, Crook DW, Timofte D.

J Antimicrob Chemother. 2017 Dec 8. doi: 10.1093/jac/dkx456. [Epub ahead of print]

10.

Evaluation of Whole-Genome Sequencing for Mycobacterial Species Identification and Drug Susceptibility Testing in a Clinical Setting: a Large-Scale Prospective Assessment of Performance against Line Probe Assays and Phenotyping.

Quan TP, Bawa Z, Foster D, Walker T, Del Ojo Elias C, Rathod P; MMM Informatics Group, Iqbal Z, Bradley P, Mowbray J, Walker AS, Crook DW, Wyllie DH, Peto TEA, Smith EG.

J Clin Microbiol. 2018 Jan 24;56(2). pii: e01480-17. doi: 10.1128/JCM.01480-17. Print 2018 Feb.

11.

mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015.

AbuOun M, Stubberfield EJ, Duggett NA, Kirchner M, Dormer L, Nunez-Garcia J, Randall LP, Lemma F, Crook DW, Teale C, Smith RP, Anjum MF.

J Antimicrob Chemother. 2017 Oct 1;72(10):2745-2749. doi: 10.1093/jac/dkx286.

PMID:
29091227
12.

High Rates of Human Fecal Carriage of mcr-1-Positive Multidrug-Resistant Enterobacteriaceae Emerge in China in Association With Successful Plasmid Families.

Zhong LL, Phan HTT, Shen C, Vihta KD, Sheppard AE, Huang X, Zeng KJ, Li HY, Zhang XF, Patil S, Crook DW, Walker AS, Xing Y, Lin JL, Feng LQ, Doi Y, Xia Y, Stoesser N, Tian GB.

Clin Infect Dis. 2018 Feb 10;66(5):676-685. doi: 10.1093/cid/cix885.

PMID:
29040419
13.

Aiming for zero tuberculosis transmission in low-burden countries.

Marais BJ, Walker TM, Cirillo DM, Raviglione M, Abubakar I, van der Werf MJ, Boehme C, Niemann S, Castro KG, Zumla A, Sintchenko V, Crook DW.

Lancet Respir Med. 2017 Nov;5(11):846-848. doi: 10.1016/S2213-2600(17)30382-X. Epub 2017 Oct 13. No abstract available.

PMID:
29033268
14.

Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches.

George S, Pankhurst L, Hubbard A, Votintseva A, Stoesser N, Sheppard AE, Mathers A, Norris R, Navickaite I, Eaton C, Iqbal Z, Crook DW, Phan HTT.

Microb Genom. 2017 Jun 9;3(8):e000118. doi: 10.1099/mgen.0.000118. eCollection 2017 Aug.

15.

Covert dissemination of carbapenemase-producing Klebsiella pneumoniae (KPC) in a successfully controlled outbreak: long- and short-read whole-genome sequencing demonstrate multiple genetic modes of transmission.

Martin J, Phan HTT, Findlay J, Stoesser N, Pankhurst L, Navickaite I, De Maio N, Eyre DW, Toogood G, Orsi NM, Kirby A, Young N, Turton JF, Hill RLR, Hopkins KL, Woodford N, Peto TEA, Walker AS, Crook DW, Wilcox MH.

J Antimicrob Chemother. 2017 Nov 1;72(11):3025-3034. doi: 10.1093/jac/dkx264.

16.

Whole-genome sequencing identifies nosocomial transmission of extra-pulmonary M. tuberculosis-response.

Walker TM, Crook DW, Peto TEA, Conlon CP.

QJM. 2017 Sep 1;110(9):613. doi: 10.1093/qjmed/hcx116. No abstract available.

PMID:
28911030
17.

Epidemiology of Clostridium difficile in infants in Oxfordshire, UK: Risk factors for colonization and carriage, and genetic overlap with regional C. difficile infection strains.

Stoesser N, Eyre DW, Quan TP, Godwin H, Pill G, Mbuvi E, Vaughan A, Griffiths D, Martin J, Fawley W, Dingle KE, Oakley S, Wanelik K, Finney JM, Kachrimanidou M, Moore CE, Gorbach S, Riley TV, Crook DW, Peto TEA, Wilcox MH, Walker AS; Modernising Medical Microbiology Informatics Group (MMMIG).

PLoS One. 2017 Aug 16;12(8):e0182307. doi: 10.1371/journal.pone.0182307. eCollection 2017.

18.

Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli.

Stoesser N, Sheppard AE, Peirano G, Anson LW, Pankhurst L, Sebra R, Phan HTT, Kasarskis A, Mathers AJ, Peto TEA, Bradford P, Motyl MR, Walker AS, Crook DW, Pitout JD.

Sci Rep. 2017 Jul 19;7(1):5917. doi: 10.1038/s41598-017-06256-2.

19.

Comparison of Control of Clostridium difficile Infection in Six English Hospitals Using Whole-Genome Sequencing.

Eyre DW, Fawley WN, Rajgopal A, Settle C, Mortimer K, Goldenberg SD, Dawson S, Crook DW, Peto TEA, Walker AS, Wilcox MH.

Clin Infect Dis. 2017 Aug 1;65(3):433-441. doi: 10.1093/cid/cix338.

20.

Staphylococcus aureus in critical care - Authors' reply.

Price JR, Crook DW, Walker AS, Peto TEA, Llewelyn MJ, Paul J.

Lancet Infect Dis. 2017 Jun;17(6):580-581. doi: 10.1016/S1473-3099(17)30269-4. No abstract available.

PMID:
28555579
21.

Mortality risks associated with emergency admissions during weekends and public holidays: an analysis of electronic health records.

Walker AS, Mason A, Quan TP, Fawcett NJ, Watkinson P, Llewelyn M, Stoesser N, Finney J, Davies J, Wyllie DH, Crook DW, Peto TEA.

Lancet. 2017 Jul 1;390(10089):62-72. doi: 10.1016/S0140-6736(17)30782-1. Epub 2017 May 9.

22.

Molecular Diagnosis of Orthopedic-Device-Related Infection Directly from Sonication Fluid by Metagenomic Sequencing.

Street TL, Sanderson ND, Atkins BL, Brent AJ, Cole K, Foster D, McNally MA, Oakley S, Peto L, Taylor A, Peto TEA, Crook DW, Eyre DW.

J Clin Microbiol. 2017 Aug;55(8):2334-2347. doi: 10.1128/JCM.00462-17. Epub 2017 May 10.

23.

Whole-Genome Sequencing Reveals the Contribution of Long-Term Carriers in Staphylococcus aureus Outbreak Investigation.

Gordon NC, Pichon B, Golubchik T, Wilson DJ, Paul J, Blanc DS, Cole K, Collins J, Cortes N, Cubbon M, Gould FK, Jenks PJ, Llewelyn M, Nash JQ, Orendi JM, Paranthaman K, Price JR, Senn L, Thomas HL, Wyllie S, Crook DW, Peto TEA, Walker AS, Kearns AM.

J Clin Microbiol. 2017 Jul;55(7):2188-2197. doi: 10.1128/JCM.00363-17. Epub 2017 May 3.

24.

Clostridium difficile in England: can we stop washing our hands? - Authors' reply.

Eyre DW, Dingle KE, Didelot X, Quan TP, Peto TEA, Wilcox MH, Walker AS, Crook DW.

Lancet Infect Dis. 2017 May;17(5):478-479. doi: 10.1016/S1473-3099(17)30185-8. No abstract available.

PMID:
28447949
25.

The relative importance of large problems far away versus small problems closer to home: insights into limiting the spread of antimicrobial resistance in England.

Donker T, Henderson KL, Hopkins KL, Dodgson AR, Thomas S, Crook DW, Peto TEA, Johnson AP, Woodford N, Walker AS, Robotham JV.

BMC Med. 2017 Apr 27;15(1):86. doi: 10.1186/s12916-017-0844-2.

26.

Enhanced Klebsiella pneumoniae Carbapenemase Expression from a Novel Tn4401 Deletion.

Cheruvanky A, Stoesser N, Sheppard AE, Crook DW, Hoffman PS, Weddle E, Carroll J, Sifri CD, Chai W, Barry K, Ramakrishnan G, Mathers AJ.

Antimicrob Agents Chemother. 2017 May 24;61(6). pii: e00025-17. doi: 10.1128/AAC.00025-17. Print 2017 Jun.

27.

WGS to predict antibiotic MICs for Neisseria gonorrhoeae.

Eyre DW, De Silva D, Cole K, Peters J, Cole MJ, Grad YH, Demczuk W, Martin I, Mulvey MR, Crook DW, Walker AS, Peto TEA, Paul J.

J Antimicrob Chemother. 2017 Jul 1;72(7):1937-1947. doi: 10.1093/jac/dkx067.

28.

Tuberculosis is changing.

Walker TM, Cruz ALG, Peto TE, Smith EG, Esmail H, Crook DW.

Lancet Infect Dis. 2017 Apr;17(4):359-361. doi: 10.1016/S1473-3099(17)30123-8. Epub 2017 Mar 13. No abstract available.

PMID:
28298254
29.

Same-Day Diagnostic and Surveillance Data for Tuberculosis via Whole-Genome Sequencing of Direct Respiratory Samples.

Votintseva AA, Bradley P, Pankhurst L, Del Ojo Elias C, Loose M, Nilgiriwala K, Chatterjee A, Smith EG, Sanderson N, Walker TM, Morgan MR, Wyllie DH, Walker AS, Peto TEA, Crook DW, Iqbal Z.

J Clin Microbiol. 2017 May;55(5):1285-1298. doi: 10.1128/JCM.02483-16. Epub 2017 Mar 8.

30.

Multi-site and nasal swabbing for carriage of Staphylococcus aureus: what does a single nose swab predict?

Young BC, Votintseva AA, Foster D, Godwin H, Miller RR, Anson LW, Walker AS, Peto TEA, Crook DW, Knox K.

J Hosp Infect. 2017 Jul;96(3):232-237. doi: 10.1016/j.jhin.2017.01.015. Epub 2017 Jan 30.

31.

The Hospital Water Environment as a Reservoir for Carbapenem-Resistant Organisms Causing Hospital-Acquired Infections-A Systematic Review of the Literature.

Kizny Gordon AE, Mathers AJ, Cheong EYL, Gottlieb T, Kotay S, Walker AS, Peto TEA, Crook DW, Stoesser N.

Clin Infect Dis. 2017 May 15;64(10):1435-1444. doi: 10.1093/cid/cix132. Review.

PMID:
28200000
32.

Contribution to Clostridium Difficile Transmission of Symptomatic Patients With Toxigenic Strains Who Are Fecal Toxin Negative.

Mawer DPC, Eyre DW, Griffiths D, Fawley WN, Martin JSH, Quan TP, Peto TEA, Crook DW, Walker AS, Wilcox MH.

Clin Infect Dis. 2017 May 1;64(9):1163-1170. doi: 10.1093/cid/cix079.

33.

Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays.

Ajileye A, Alvarez N, Merker M, Walker TM, Akter S, Brown K, Moradigaravand D, Schön T, Andres S, Schleusener V, Omar SV, Coll F, Huang H, Diel R, Ismail N, Parkhill J, de Jong BC, Peto TE, Crook DW, Niemann S, Robledo J, Smith EG, Peacock SJ, Köser CU.

Antimicrob Agents Chemother. 2017 Mar 24;61(4). pii: e02169-16. doi: 10.1128/AAC.02169-16. Print 2017 Apr.

34.

Effects of control interventions on Clostridium difficile infection in England: an observational study.

Dingle KE, Didelot X, Quan TP, Eyre DW, Stoesser N, Golubchik T, Harding RM, Wilson DJ, Griffiths D, Vaughan A, Finney JM, Wyllie DH, Oakley SJ, Fawley WN, Freeman J, Morris K, Martin J, Howard P, Gorbach S, Goldstein EJC, Citron DM, Hopkins S, Hope R, Johnson AP, Wilcox MH, Peto TEA, Walker AS, Crook DW; Modernising Medical Microbiology Informatics Group.

Lancet Infect Dis. 2017 Apr;17(4):411-421. doi: 10.1016/S1473-3099(16)30514-X. Epub 2017 Jan 25.

35.

Re-emergence of methicillin susceptibility in a resistant lineage of Staphylococcus aureus.

Ledda A, Price JR, Cole K, Llewelyn MJ, Kearns AM, Crook DW, Paul J, Didelot X.

J Antimicrob Chemother. 2017 May 1;72(5):1285-1288. doi: 10.1093/jac/dkw570.

36.

Chromosomal Integration of the Klebsiella pneumoniae Carbapenemase Gene, blaKPC, in Klebsiella Species Is Elusive but Not Rare.

Mathers AJ, Stoesser N, Chai W, Carroll J, Barry K, Cherunvanky A, Sebra R, Kasarskis A, Peto TE, Walker AS, Sifri CD, Crook DW, Sheppard AE.

Antimicrob Agents Chemother. 2017 Feb 23;61(3). pii: e01823-16. doi: 10.1128/AAC.01823-16. Print 2017 Mar.

37.

Whole-genome sequencing identifies nosocomial transmission of extra-pulmonary M. tuberculosis.

Walker TM, Crook DW, Peto TE, Conlon CP.

QJM. 2016 Dec;109(12):819-820. doi: 10.1093/qjmed/hcw202. Epub 2016 Dec 22. No abstract available.

38.

Transmission of Staphylococcus aureus between health-care workers, the environment, and patients in an intensive care unit: a longitudinal cohort study based on whole-genome sequencing.

Price JR, Cole K, Bexley A, Kostiou V, Eyre DW, Golubchik T, Wilson DJ, Crook DW, Walker AS, Peto TEA, Llewelyn MJ, Paul J; Modernising Medical Microbiology informatics group.

Lancet Infect Dis. 2017 Feb;17(2):207-214. doi: 10.1016/S1473-3099(16)30413-3. Epub 2016 Nov 16.

39.

Whole genome sequencing for M/XDR tuberculosis surveillance and for resistance testing.

Walker TM, Merker M, Kohl TA, Crook DW, Niemann S, Peto TE.

Clin Microbiol Infect. 2017 Mar;23(3):161-166. doi: 10.1016/j.cmi.2016.10.014. Epub 2016 Oct 24. Review.

40.

Whole-genome sequencing to determine transmission of Neisseria gonorrhoeae: an observational study.

De Silva D, Peters J, Cole K, Cole MJ, Cresswell F, Dean G, Dave J, Thomas DR, Foster K, Waldram A, Wilson DJ, Didelot X, Grad YH, Crook DW, Peto TE, Walker AS, Paul J, Eyre DW.

Lancet Infect Dis. 2016 Nov;16(11):1295-1303. doi: 10.1016/S1473-3099(16)30157-8. Epub 2016 Jul 12.

41.

Complete Sequencing of Plasmids Containing blaOXA-163 and blaOXA-48 in Escherichia coli Sequence Type 131.

Stoesser N, Sheppard AE, Peirano G, Sebra R, Lynch T, Anson L, Kasarskis A, Motyl MR, Crook DW, Pitout JD.

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6948-6951. doi: 10.1128/AAC.01130-16. Print 2016 Nov.

42.

Maternal colonization with Streptococcus agalactiae and associated stillbirth and neonatal disease in coastal Kenya.

Seale AC, Koech AC, Sheppard AE, Barsosio HC, Langat J, Anyango E, Mwakio S, Mwarumba S, Morpeth SC, Anampiu K, Vaughan A, Giess A, Mogeni P, Walusuna L, Mwangudzah H, Mwanzui D, Salim M, Kemp B, Jones C, Mturi N, Tsofa B, Mumbo E, Mulewa D, Bandika V, Soita M, Owiti M, Onzere N, Walker AS, Schrag SJ, Kennedy SH, Fegan G, Crook DW, Berkley JA.

Nat Microbiol. 2016 May 23;1(7):16067. doi: 10.1038/nmicrobiol.2016.67.

43.

Identifying lineage effects when controlling for population structure improves power in bacterial association studies.

Earle SG, Wu CH, Charlesworth J, Stoesser N, Gordon NC, Walker TM, Spencer CCA, Iqbal Z, Clifton DA, Hopkins KL, Woodford N, Smith EG, Ismail N, Llewelyn MJ, Peto TE, Crook DW, McVean G, Walker AS, Wilson DJ.

Nat Microbiol. 2016 Apr 4;1:16041. doi: 10.1038/nmicrobiol.2016.41.

44.

First Report of blaIMP-14 on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131.

Stoesser N, Sheppard AE, Peirano G, Sebra RP, Lynch T, Anson LW, Kasarskis A, Motyl MR, Crook DW, Pitout JD.

Antimicrob Agents Chemother. 2016 Jul 22;60(8):5068-71. doi: 10.1128/AAC.00840-16. Print 2016 Aug.

45.

Natural mutations in a Staphylococcus aureus virulence regulator attenuate cytotoxicity but permit bacteremia and abscess formation.

Das S, Lindemann C, Young BC, Muller J, Österreich B, Ternette N, Winkler AC, Paprotka K, Reinhardt R, Förstner KU, Allen E, Flaxman A, Yamaguchi Y, Rollier CS, van Diemen P, Blättner S, Remmele CW, Selle M, Dittrich M, Müller T, Vogel J, Ohlsen K, Crook DW, Massey R, Wilson DJ, Rudel T, Wyllie DH, Fraunholz MJ.

Proc Natl Acad Sci U S A. 2016 May 31;113(22):E3101-10. doi: 10.1073/pnas.1520255113. Epub 2016 May 16.

46.

Corrigendum: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis.

Bradley P, Gordon NC, Walker TM, Dunn L, Heys S, Huang B, Earle S, Pankhurst LJ, Anson L, de Cesare M, Piazza P, Votintseva AA, Golubchik T, Wilson DJ, Wyllie DH, Diel R, Niemann S, Feuerriegel S, Kohl TA, Ismail N, Omar SV, Smith EG, Buck D, McVean G, Walker AS, Peto TE, Crook DW, Iqbal Z.

Nat Commun. 2016 Apr 20;7:11465. doi: 10.1038/ncomms11465. No abstract available.

47.

Nested Russian Doll-Like Genetic Mobility Drives Rapid Dissemination of the Carbapenem Resistance Gene blaKPC.

Sheppard AE, Stoesser N, Wilson DJ, Sebra R, Kasarskis A, Anson LW, Giess A, Pankhurst LJ, Vaughan A, Grim CJ, Cox HL, Yeh AJ; Modernising Medical Microbiology (MMM) Informatics Group, Sifri CD, Walker AS, Peto TE, Crook DW, Mathers AJ.

Antimicrob Agents Chemother. 2016 May 23;60(6):3767-78. doi: 10.1128/AAC.00464-16. Print 2016 Jun.

48.

Evolutionary History of the Global Emergence of the Escherichia coli Epidemic Clone ST131.

Stoesser N, Sheppard AE, Pankhurst L, De Maio N, Moore CE, Sebra R, Turner P, Anson LW, Kasarskis A, Batty EM, Kos V, Wilson DJ, Phetsouvanh R, Wyllie D, Sokurenko E, Manges AR, Johnson TJ, Price LB, Peto TE, Johnson JR, Didelot X, Walker AS, Crook DW; Modernizing Medical Microbiology Informatics Group (MMMIG).

MBio. 2016 Mar 22;7(2):e02162. doi: 10.1128/mBio.02162-15.

49.

Capsular Typing Method for Streptococcus agalactiae Using Whole-Genome Sequence Data.

Sheppard AE, Vaughan A, Jones N, Turner P, Turner C, Efstratiou A, Patel D; Modernising Medical Microbiology Informatics Group, Walker AS, Berkley JA, Crook DW, Seale AC.

J Clin Microbiol. 2016 May;54(5):1388-90. doi: 10.1128/JCM.03142-15. Epub 2016 Mar 9.

50.

Global Genomic Epidemiology of Salmonella enterica Serovar Typhimurium DT104.

Leekitcharoenphon P, Hendriksen RS, Le Hello S, Weill FX, Baggesen DL, Jun SR, Ussery DW, Lund O, Crook DW, Wilson DJ, Aarestrup FM.

Appl Environ Microbiol. 2016 Apr 4;82(8):2516-26. doi: 10.1128/AEM.03821-15. Print 2016 Apr.

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